EMD Millipore Protein Blotting Handbook Request
Get valuable tips and tricks to help improve your Western blotting, making the process easier and more effective. Just fill out the form below and you can download this essential guide today — or request a printed version.
Included in the handbook are tips like:
- Tip: Phosphatases in the blocking solution may dephosphorylate blotted proteins
- Tip: Do not use sodium azide in the buffers as it inhibits HRP activity
- Recommended: Do not let the blot dry out at any time during and after blocking
- Tip: If more than one blot is placed in a container, insufficient buffer volume will cause the blots to stick together
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