Search results for "New England Biolabs Inc "
New England Biolabs, Inc. NEB Stable Competent E. coli (High Efficiency) – 20 x 0.05 ml/tube
Chemically competent E. coli cells suitable for high efficiency transformation and isolation of plasmid clones containing repeat elements.
New England Biolabs, Inc. NEB 10-beta Competent E. coli (High Efficiency) – 20 x 0.05 ml/tube
NEB 10-beta Competent E. coli is a derivative of the popular DH10B. It is T1 phage resistant and endonuclease I (endA1) deficient for high- quality plasmid preparations.
New England Biolabs, Inc. β-N-Acetylglucosaminidase S - 500 units
b-N-Acetylglucosaminidase S is an exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing b-N-Acetylglucosamine residues from oligosaccharides.
New England Biolabs, Inc. NEBNext Q5 Hot Start HiFi PCR Master Mix – 50 reactions
NEBNext Q5 Hot Start HiFi PCR Master Mix is the original hot start NEBNext formulation of Q5 High-Fidelity DNA polymerase. This formulation is also included as a component in all of the original NEBNext Ultra kits, for DNA and RNA library preparation. However, the NEBNext Ultra II Q5 Master Mix is now available, and this more recent hot start formulation further improves the uniformity of amplification of libraries, including superior performance with GC-rich regions.
New England Biolabs, Inc. ApoI-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence R^AATTY. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. Apyrase – 10 units
Apyrase (recombinant, E. coli) is a highly active ATP-diphosphohydrolase that catalyses the sequential hydrolysis of ATP to ADP and ADP to AMP releasing inorganic phosphate. It is a recombinant version of one of several isoforms of apyrase. It can also hydrolyse 5' tri- and diphosphate ribonucleosides and deoxyribonucleosides to their respective 5' monophosphates. Apyrase can catalyse the conversion of 5' triphosphorylated RNA to 5' monophosphorylated RNA.
New England Biolabs, Inc. HiScribe™ T7 ARCA mRNA Kit – 20 reactions
Most eukaryotic mRNAs require a 7-methyl guanosine (m7G) cap structure at the 5' end and a Poly(A) tail at the 3 end to be efficiently translated. By using a DNA template encoding a poly(A) tail, the HiScribe T7 ARCA mRNA Kit can be used to synthesize capped and tailed mRNAs. The cap structure is added to the mRNA by co-transcriptional incorporation of Anti-Reverse Cap Analog (ARCA) using T7 RNA Polymerase. Poly(A) tail is incorporated during the transcription reaction. The kit also includes DNase I and LiCl for DNA template removal and quick mRNA purification. Additionally, the kit is capable of partial incorporation of modified UTP and CTP (up to 50% each) without affecting the mRNA yield significantly. By using a DNA template encoding a poly(A) tail, capped and tailed modified mRNA can be synthesized in a single reaction in 30 minutes. mRNAs synthesized with the kit can be used for cell transfection, microinjection, in vitro translation and RNA vaccines.
New England Biolabs, Inc. NEBuilder HiFi DNA Assembly Master Mix – 250 reactions
NEBuilder HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly. It allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. Used to assemble either ss oligonucleotides or different sizes of DNA frags with varied overlaps. Has utility in synbio community, as well as those interested in one-step cloning of multiple fragments due to its ease of use, flexibility and simple MM format. The rxn includes different enzymes that work together in the same buffer: The exonuclease creates single-stranded 3' overhangs that facilitate the annealing of fragments that share complementarity at one end (the overlap region): a polymerase fills in gaps within each annealed fragment; DNA ligase seals nicks in the assembled DNA. End result is a ds fully sealed DNA molecule that can serve as template for PCR, RCA or a variety of other molecular biology applications, including direct transformation of E coli.
New England Biolabs, Inc. NEBNext Microbiome DNA Enrichment Kit – 24 reactions
The NEBNext Microbiome DNA Enrichment Kit facilitates enrichment of microbial DNA from samples containing methylated host DNA (including human), by selective binding and removal of the CpG-methylated host DNA. Importantly, microbial diversity remains intact after enrichment.
New England Biolabs, Inc. Quick-Load Purple 1 kb DNA Ladder – 1.25 ml
Quick-Load Purple 1 kb DNA Ladder is a pre-mixed, ready-to-load molecular weight DNA marker containing one tracking dye. Tracking dye does not leave a shadow under UV light. Quick-Load Purple 1 kb DNA Ladder consists of proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 0.5 to 10 kb. The 3 kb fragment has increased intensity to serve as reference band.
New England Biolabs, Inc. NEBNext Ultra™ II Ligation Module – 96 reactions
This module is part of the Ultra II workflow, and is optimized for use with the NEBNext Ultra II End Repair/dA-Tailing Module, for Illumina-compatible library construction. The NEBNext Ultra II Ligation Module is optimized for use with the NEBNext Ultra II End Repair/dA-Tailing Module or the NEBNext Ultra II FS DNA Module. This module is also compatible with some Oxford Nanopore Technologies workflows.
New England Biolabs, Inc. Bst 2.0 WarmStart DNA Polymerase (Glycerol-free) - 8000 units
Bst 2.0 WarmStart DNA Polymerase (Glycerol-free) is formulated without glycerol to support lyophilization, incorporation into microfluidic devices, and enable quick adoption into automation workflows. Bst 2.0 WarmStart DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment (Bst DNA Polymerase, Large Fragment) with a reversibly-bound aptamer, which inhibits polymerase activity at temperatures below 45°C. At temperatures above 45°C, the aptamer rapidly releases from the enzyme with no special activation step needed to activate the polymerase. Bst 2.0 WarmStart DNA Polymerase contains 5' to 3' DNA polymerase activity and strong strand-displacement activity but lacks 5' to 3' exonuclease activity. Bst 2.0 WarmStart DNA Polymerase displays improved amplification speed, yield, salt tolerance, and thermostability compared to wild-type Bst DNA Polymerase, Large Fragment.
New England Biolabs, Inc. EnGen SpRY Cas9 - 500 pmol
EnGen SpRY Cas9 from Streptococcus pyogenes is an engineered, RNA-guided, DNA endonuclease that catalyzes site-specific cleavage of double-stranded DNA (dsDNA).
New England Biolabs, Inc. LyoPrime Luna Probe One-Step RT-qPCR Mix with UDG - 96 rxns
The LyoPrime Luna Probe One-Step RT-qPCR Mix with UDG is offered in a lyophilized format, allowing for ambient/room temperature shipping and storage prior to use. The mix contains all necessary components for one-step RT-qPCR except primers, probe(s), and RNA sample. It can be reconstituted by simply adding water to a 2-4X concentration, allowing for increased sample input. Performance in multiplexing applications has been optimized, with sensitive, linear detection achieved for up to 5 targets across a range of inputs. It is available in 2 x 60 reaction glass vials or a 96-well plate (1 reaction per well).
New England Biolabs, Inc. LyoPrime WarmStart™ Fluorescent LAMP/RT-LAMP Mix (with UDG) - 100 rxns
The LyoPrime WarmStart Fluorescent LAMP/RT-LAMP Mix (with UDG) is offered in a lyophilized format, allowing for ambient/room temperature shipping and storage prior to use. It is a simple, one-step solution for real-time detection of LAMP/RT-LAMP reactions via fluorescence. LAMP and RT-LAMP are commonly used isothermal amplification techniques that provide rapid detection of a target nucleic acid using LAMP-specific primers and a strand-displacing DNA polymerase. Featuring a blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized buffer solution, this mix offers improved performance. LAMP Fluorescent Dye at 0.5X concentration is included to enable detection of amplification via real time fluorescence. Antarctic Thermolabile UDG and dUTP reduce the risk of carryover contamination between reactions. It is available in 2 x 50 reaction glass vials or a 96-well plate (1 reaction per well).