Restriction Enzymes
Restriction Enzymes
New England Biolabs, Inc. ApoI-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence R^AATTY. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. BclI-HF – 15000 units
A high fidelity restriction endonuclease that recognizes the sequence T^GATCA. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
New England Biolabs, Inc. PaqCI - 200 units
PaqCI is a Type IIS restriction enzyme with a 7 basepair recognition sequence: CACCTGC(4/8). It is sourced from an E. coli strain that carries the modified cloned PaqCI gene from Paucibacter aquatile. PaqCI requires multiple sites for efficient cleavage -- PaqCI Activator is included for optimal cleavage. This enzyme is supplied with NEB's rCutSmart Buffer and is heat inactivated.
New England Biolabs, Inc. PluTI – 500 units
A restriction endonuclease that recognizes the sequence GGCGC^C
New England Biolabs, Inc. Exonuclease V (RecBCD) – 5000 units
Exonuclease V, a RecBCD complex from E. coli has several different enzyme activities, including an ATP-dependent single-stranded DNA endonuclease activity, ss- and ds- DNA exonuclease activity. The hydrolysis in each case is bi-directional (from both the 3' and 5' ends) and processive, producing oligonucleotides. All Exonuclease V activities have divalent cation requirements. Mg2+ is required for the exonuclease activity, while Ca2+ inhibits the exonuclease activity and allows double-stranded DNA unwinding (helicase activity) without hydrolysis.
New England Biolabs, Inc. BmtI-HF – 300 units
A high fidelity restriction endonuclease that recognizes the sequence G_CTAG^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. XRN-1 – 100 units
XRN-1 is highly processive 53 exoribonuclease, requiring 5 monophosphate. It also acts on 5' monophosphate ssDNA with greatly reduced efficiency.
New England Biolabs, Inc. AfeI – 1000 units
A restriction endonuclease that recognizes the sequence AGC_^GCT.
New England Biolabs, Inc. BspQI – 500 units
A restriction endonuclease that recognizes the sequence GCTCTTCN^NNN_.
New England Biolabs, Inc. T7 Endonuclease I – 250 units
T7 Endonuclease I recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA and more slowly, nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5' to the mismatch. The protein is the product of T7 gene 3.
New England Biolabs, Inc. CspCI – 500 units
A restriction endonuclease that recognizes the sequence _NN^NNNNNNNNNNNCAANNNNNGTGGNNNNNNNNNN_NN^.
New England Biolabs, Inc. Nb.BsrDI – 1000 units
A restriction endonuclease that recognizes the sequence GCAATG_NN.
New England Biolabs, Inc. Nb.BtsI – 1000 units
A restriction endonuclease that recognizes the sequence GCAGTG_NN.
New England Biolabs, Inc. HpyAV – 500 units
A restriction endonuclease that recognizes the sequence CCTTCNNNNN_N^.
New England Biolabs, Inc. Nt.BsmAI – 500 units
A restriction endonuclease that recognizes the sequence GTCTCN^N.