K-Blue Advanced Plus is a one-bottle stabilized chromogenic substrate for use with horseradish peroxidase immunoassays. It contains both 3,3’,5,5’ tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2). K-Blue Advanced Plus develops a deep blue color in the presence of peroxidase labeled conjugate. This substrate has been optimized for use with microwell assays, especially ELISA. K-Blue Advanced Plus is not applicable for use with assays requiring a precipitating substrate. The substrate offers long-term stability and high activity. K-Blue Advanced Plus is compliant with Annex XVII for REACH and contains no hazardous substances at or above published reporting levels. Stable for a minimum of 24-months when stored at 2-8C.