Crosslinking, Labeling and Protein Modification
Crosslinking, Labeling and Protein Modification
New England Biolabs, Inc. α-Lytic Protease - 100 µg
alpha-Lytic Protease (aLP) cleaves after Threonine (T), Alanine (A), Serine (S) and Valine (V) residues. Its specificity makes it an orthogonal and alternative protease to others commonly used in proteomics applications, including trypsin and chymotrypsin. Peptides generated by aLP are of similar average length to those of Trypsin.
New England Biolabs, Inc. SNAP-Surface 649 – 50 nmol
SNAP-Surface 649 is a photostable fluorescent substrate that can be used to label SNAP-tag fusion proteins on cell surfaces and in solution.
New England Biolabs, Inc. SNAP-Cell Oregon Green – 50 nmol
SNAP-Cell Oregon Green is a photostable green fluorescent substrate that can be used to label SNAP-tag fusion proteins inside living cells or in vitro. Conjugates of Oregon Green are more photostable than those of fluorescein, and their fluorescence properties are essentially pH insensitive in the physiological pH range.
New England Biolabs, Inc. TEV Protease – 1000 units
TEV Protease is a highly specific cysteine protease that recognizes the amino-acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) and cleaves between the Gln and Gly/Ser residues. It is often used for the removal of affinity purification tags such as maltose-binding protein (MBP) or poly-histidine from fusion proteins. TEV Protease has a 7xHis-tag for easy removal from a reaction using nickel affinity resins and has been engineered to improve thermal stability and decrease autolysis.
New England Biolabs, Inc. α-Lytic Protease - 20 µg
alpha-Lytic Protease (aLP) cleaves after Threonine (T), Alanine (A), Serine (S) and Valine (V) residues. Its specificity makes it an orthogonal and alternative protease to others commonly used in proteomics applications, including trypsin and chymotrypsin. Peptides generated by aLP are of similar average length to those of Trypsin.
New England Biolabs, Inc. O-Glycoprotease -200 reactions
O-Glycoprotease is a highly specific protease that cleaves the peptide bonds of a glycoprotein or glycopeptide immediately N-terminal to a serine or threonine residue containing a mucin-type O-linked glycan with or without sialylation. O-Glycoprotease has a 6xHis-tag for easy removal from a reaction using nickel affinity resins.
New England Biolabs, Inc. Endo D – 1500 units
Endo D is a recombinant glycosidase, which cleaves within the chitobiose core of paucimannose N-linked glycans, with or without extensions in the antennae. Endo D is tagged with a chitin binding domain (CBD) for easy removal from a reaction, and is supplied glycerol-free for optimal performance in HPLC and MS- intensive methods.
New England Biolabs, Inc. SNAP-Cell 647-SiR – 30 nmol
SNAP-Cell 647-SiR is a far-red fluorescent substrate that can be used to label SNAP-tag fusion proteins inside living cells, on cell surfaces, or in vitro.
New England Biolabs, Inc. IdeZ Protease (IgG-specific) – 4000 units
IdeZ Protease (IgG-specific) is a recombinant antibody specific protease that recognizes all human, sheep, monkey, and rabbit IgG subclasses, cleaving specifically at a single recognition site below the hinge region, to yield a homogenous pool of F(ab)2 and Fc fragments. IdeZ Protease more effectively cleaves murine IgG2a than IdeS.
New England Biolabs, Inc. CLIP-Cell™ 505 – 50 nmol
CLIP-Cell 505 is a green fluorescent substrate that can be used to label CLIP-tag fusion proteins inside living cells, on cell surfaces or in vitro.