Competent Cells
Competent Cells
Thermo Scientific™ DH5α Competent Cells; High Efficiency
High efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors
Thermo Scientific™ DH10B Competent Cells; High Efficiency
High efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently.
MilliporeSigma™ Novagen™ NovaBlue GigaSingles™ Competent Cells
NovaBlue GigaSingles™ Competent Cells produce greater than 1 x 10⁹ colonies/μg plasmid DNA for cloning applications requiring high-efficiency transformations.
Thermo Scientific™ DH5α Competent Cells
Chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of cDNA libraries.
MilliporeSigma™ Novagen™ HMS174 Competent Cells
HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.
Thermo Scientific™ BL21(DE3) Competent Cells
Suitable for the expression of non-toxic heterologous genes. The strain contains the lambda DE3 prophage that carries the gene for T7 RNA polymerase under control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG.
MilliporeSigma™ Novagen™ HMS174 (DE3) pLysS Competent Cells
HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. T7 lysozyme expression suppresses basal T7 expression.
MilliporeSigma™ Novagen™ HMS174 (DE3) Competent Cells
HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.
Clontech Labs 3P Stellar™ Competent Cells (dam-/dcm-), 10 transformations
Stellar Competent Cells (dam-/dcm-) are an E. coli HST04 strain that lacks the genetic factors (dam and dcm) necessary for the methylation of DNA. Plasmids prepared using this product can be cut by restriction enzymes which are normally blocked by dam or dcm methylation. A dam/recA double mutation is lethal, so the parent strain is recA+. Therefore, it follows that transformation of extracellular DNA with repeat sequences can result in recombination by recA. This product is not a suitable cloning host. For transformation, it is highly recommended to use this product for constructing plasmids as mentioned above. A pUC19 vector is provided as well as SOC Medium. 10 transformations
MilliporeSigma™ Novagen™ BL21(DE3) Singles™ Competent Cells
BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system.
MilliporeSigma™ Novagen™ BL21(DE3) Competent Cells
Achieve greater efficiency, reproducibility and convenience with Novagen prepared competent cells. BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high-level protein expression using a T7 RNA polymerase-IPTG induction system.
BIOSEARCH TECHNOLOGIES INC Endura Electrocompetent Cells, 12 Rxns (DUOs)Clone and maintain unstable or repetitive sequences and lentiviral libraries with high efficiency. Ideal for GeCKO CRISPR library transformation.
Lucigens Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be propagated stably.
BIOSEARCH TECHNOLOGIES INC Endura Electrocompetent Cells, 24 Rxns (DUOs)Clone and maintain unstable or repetitive sequences and lentiviral libraries with high efficiency. Ideal for GeCKO CRISPR library transformation.
Lucigens Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be propagated stably.
BIOSEARCH TECHNOLOGIES INC Endura Chemically Competent Cells, 12 Rxns (DUOs)Clone and maintain unstable or repetitive sequences and lentiviral libraries with high efficiency. Ideal for GeCKO CRISPR library transformation.
Lucigens Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be propagated stably.
BIOSEARCH TECHNOLOGIES INC Endura Chemically Competent Cells, 24 Rxns (DUOs)Clone and maintain unstable or repetitive sequences and lentiviral libraries with high efficiency. Ideal for GeCKO CRISPR library transformation.
Clone repetitive sequences and lentiviral librariesStabilize direct repeats and create lentiviral constructsGenerate CRISPR-GeCKO-Libraries-Endura lentiviral guide RNA librariesRecommended in CRISPR GeCKO library protocolsChoose electrocompetent or chemically competent cellsHighest efficiency commercially available cells for lentiviral cloningEndura™ Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube) or DUOs (2 transformations per tube) as indicated. Endura Electrocompetent cells are provided in DUOs format only. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.