Invitrogen™ pENTR™ 5'-TOPO™ TA Cloning™ Kit, with One Shot™ TOP10 Chemically Competent E. coli

Facilitates five-minute, one-step cloning of a Taq polymerase-amplified PCR product encoding a eukaryotic promoter of interest into a MultiSite Gateway entry vector with ≥85% efficiency

Invitrogen™ Champion™ pET151 Directional TOPO™ Expression Kit with BL21 Star™ (DE3) One Shot™ Chemically Competent E. coli

Protein of interest can reach levels greater than 50 percent of total cellular protein during expression

Invitrogen™ Champion™ pET200 Directional TOPO™ Expression Kit with BL21 Star™ (DE3) One Shot™ Chemically Competent E. coli

Protein of interest can reach levels greater than 50 percent of total cellular protein in E. coli during expression

Invitrogen™ Champion™ pET101 Directional TOPO™ Expression Kit with BL21 Star™ (DE3) One Shot™ Chemically Competent E. coli

Yields protein of interest at levels greater than 50 percent of total cellular protein in E. coli

Invitrogen™ Champion™ pET100 Directional TOPO™ Expression Kit with BL21 Star™ (DE3) One Shot™ Chemically Competent E. coli

Yields protein of interest at levels greater than 50 percent of total cellular protein in E. coli

Invitrogen™ Champion™ pET102 Directional TOPO™ Expression Kit with BL21 Star™ (DE3) One Shot™ Chemically Competent E. coli

Protein of interest can reach levels greater than 50 percent of total cellular protein in E. coli during expression

Agilent Technologies PET-11 A-D VECTORS

pET-11 a-d Vectors. Tightly controlled T7 RNA polymerase-based gene expression in E. coli. Convenient cloning sites for optimal expression of cloned protein-coding sequence. BL21(DE3) and BL21(DE3)pLysS competent cells are provided.