Nucleic Acid Introduction Reagents
Nucleic Acid Introduction Reagents
Enzo Life Sciences Nupherin™ Transfection Reagent (3 mg)
Nupherin™ dramatically enhances the performance of commercially available cationic lipids to allow high-efficiency lipofection (10-40%) of neuronal, ganglion and other difficult to transfect cells. Efficient lipofection is limited by the ability of transfected DNA to enter the nucleus – a particular obstacle in nondividing or slow dividing cells like neurons. Nupherin, a peptide based reagent that combines a non-classical nuclear localization sequence (M9) with a nucleotide binding domain, helps to transport DNA into the transfected cell's nucleus. Nupherin has been demonstrated to provide high efficiency lipofection of primary rat hippocampal neurons, chicken embryonic retinal ganglian cells, rat pheochromocytoma (PC12) cells and other recalcitrant cells. Fully compatible with cells grown on glass coverslips, in plastic plates, or in tissue-culture flasks. Formulation: Liquid. Sterile solution. Long Term Storage: -80°C.
Enzo Life Sciences Nupherin™ Transfection Reagent, (0.75mg)
Greatly enhances transfection efficiency. Use/Stability: On first use, aliquot the reagent and store at -80°C. Thawed aliquots of Nupherin™ can be frozen after use for. Long Term Storage or refrigerated for up to 1 week. Do not dilute reagent. Long Term Storage: -80°C