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ELISA Substrates and Reagents

ELISA Substrates and Reagents

Colorimetric, chemiluminescent and chemifluorescent substrates for alkaline phosphatase (AP), horseradish peroxidase (HRP) and other enzyme reporter systems; includes several sensitive formulations of TMB for kinetic, competition or sandwich ELISA.
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1 – 15 of 1,532 results
1

Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions
GSA_VA

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

EDGE
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Detection Method Colorimetric
2

Thermo Scientific™ Stop Solution for TMB Substrates
GSA_VA

Stop TMB-based ELISA detection with this 0.16M sulfuric acid for use with the ELISA substrate 3,3',5,5' - tetramethylbenzidine (TMB).

EDGE
3

Invitrogen™ ELISA Stop Solution

A ready-to-use solution intended for use as a stop solution in ELISA applications that use horseradish peroxidase and TMB substrate solution.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Ambient
Content And Storage Store at 2°C to 8°C
Form Liquid
Product Type ELISA Stop Solution
Substrate Type HRP, TMB
4

Invitrogen™ RAGE Biosimilar Recombinant Monoclonal Antibody

Recombinant Monoclonal Antibody

ENCOMPASS_PREFERRED
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Target Species Human
Host Species Artificial Control
Conjugate Unconjugated
Applications ELISA,Flow Cytometry,Functional Assay,Surface Plasmon Resonance
Form Lyophilized
Isotype IgG1
Concentration 1 mg/mL
Antigen RAGE Biosimilar
Regulatory Status RUO
Purification Method Protein A
Product Type Antibody
Formulation 25 mM histidine with 8% sucrose, 0.01% Tween 80 and no preservative; pH 6.2
Classification Recombinant Monoclonal
Primary or Secondary Primary
5

Invitrogen™ Laminin gamma-1 Recombinant Rabbit Monoclonal Antibody (7B2C2)

Rabbit Recombinant Monoclonal Antibody

ENCOMPASS_PREFERRED
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Target Species Mouse,Rat
Host Species Rabbit
Conjugate Unconjugated
Applications ELISA,Western Blot
Form Liquid
Gene Accession No. P02468
Isotype IgG
Concentration 0.22 mg/mL
Antigen Laminin gamma-1
Gene Symbols LAMC1
Regulatory Status RUO
Purification Method Protein A
Gene Alias AA408497; B2e; basement membrane protein; LAMA; LAMA1; Lama-1; LAMB2; LAMC1; Laminin A chain; laminin B2 chain; laminin gamma 1 chain; laminin S; laminin subunit alpha 1; laminin subunit alpha-1; laminin subunit gamma 1; laminin subunit gamma-1; laminin, alpha 1; laminin, beta 2; laminin, gamma 1; laminin, gamma 1 (formerly LAMB2); laminin-1; laminin-1 subunit alpha; Laminin-1 subunit gamma; laminin-10 subunit gamma; Laminin-11 subunit gamma; laminin-2 subunit gamma; Laminin-3 subunit alpha; laminin-3 subunit gamma; Laminin-4 subunit gamma; laminin-6 subunit gamma; Laminin-7 subunit gamma; laminin-8 subunit gamma; Laminin-9 subunit gamma; PTBHS; S-LAM alpha; S-LAM gamma; S-LAM-alpha; S-laminin subunit alpha; S-laminin subunit gamma
Gene LAMC1
Product Type Antibody
Gene ID (Entrez) 117036, 226519
Formulation PBS with 0.05% BSA, 50% glycerol and 0.09% sodium azide; pH 7.3
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1030-1275 of human LAMC1 (NP_002284.3).
Classification Recombinant Monoclonal
Primary or Secondary Primary
Clone 7B2C2
6

Invitrogen™ IkB alpha Recombinant Rabbit Monoclonal Antibody (1I1F7)

Rabbit Recombinant Monoclonal Antibody

ENCOMPASS_PREFERRED
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Target Species Human,Mouse,Rat
Host Species Rabbit
Conjugate Unconjugated
Applications ELISA,Western Blot
Form Liquid
Gene Accession No. P25963, Q63746, Q9Z1E3
Isotype IgG
Concentration 1.63 mg/mL
Antigen IkB alpha
Gene Symbols NFKBIA
Regulatory Status RUO
Purification Method Protein A
Gene Alias AI462015; ECI-6; ECI-6/IKBA; I kappa B-alpha; I(Kappa)B(alpha); I79_018146; IkappaB alpha; IkappaBalpha; I-kappaBalpha; I-kappa-B-alpha; Ikba; IKBalpha; IKB-alpha; Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells alpha; Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells, alpha; MAD3; MAD-3; Major histocompatibility complex enhancer-binding protein MAD3; NF kappa B inhibitor alpha; NF-kappaB inhibitor alpha; NF-kappa-B inhibitor alpha; NFKB inhibitor alpha; NFKBI; Nfkbia; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, alpha; nuclear factor of kappa light polyp gene enhancer in B-cell 1; nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha; Rel-associated pp40; REL-associated protein pp40; RL/IF-1
Gene NFKBIA
Product Type Antibody
Gene ID (Entrez) 18035, 25493, 4792
Formulation PBS with 0.05% BSA, 50% glycerol and 0.09% sodium azide; pH 7.3
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-314 of rat I-kappa-B-alpha (NP_001099190.2).
Classification Recombinant Monoclonal
Primary or Secondary Primary
Clone 1I1F7
7

Invitrogen™ CD166 (ALCAM) Biosimilar Recombinant Monoclonal Antibody

Recombinant Monoclonal Antibody

ENCOMPASS_PREFERRED
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Target Species Human
Host Species Artificial Control
Conjugate Unconjugated
Applications ELISA,Flow Cytometry,Functional Assay,Surface Plasmon Resonance
Form Lyophilized
Isotype IgG1
Concentration 1 mg/mL
Antigen CD166 (ALCAM) Biosimilar
Regulatory Status RUO
Purification Method Protein A
Product Type Antibody
Formulation 25mM histidine with 8% sucrose, 0.01% Tween 80 and no preservative; pH 6.2
Classification Recombinant Monoclonal
Primary or Secondary Primary
8

Invitrogen™ Osteopontin Biosimilar Recombinant Monoclonal Antibody

Recombinant Monoclonal Antibody

ENCOMPASS_PREFERRED
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Target Species Human
Host Species Artificial Control
Conjugate Unconjugated
Applications ELISA,Flow Cytometry,Functional Assay,Surface Plasmon Resonance
Form Lyophilized
Isotype IgG1
Concentration 1 mg/mL
Antigen Osteopontin Biosimilar
Regulatory Status RUO
Purification Method Protein A
Product Type Antibody
Formulation 25mM histidine with 8% sucrose, 0.01% Tween 80 and no preservative; pH 6.2
Classification Recombinant Monoclonal
Primary or Secondary Primary
9

Invitrogen™ IkB zeta Recombinant Rat Monoclonal Antibody (LK2NAP)

Rat Recombinant Monoclonal Antibody

ENCOMPASS_PREFERRED
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target Species Human,Mouse,Rat
Host Species Rat
Conjugate Unconjugated
Applications Flow Cytometry,Western Blot,Immunocytochemistry
Form Liquid
Gene Accession No. Q9BYH8, Q9EST8
Isotype IgG2a
Concentration 1.0 mg/mL
Antigen IkB zeta
Gene Symbols NFKBIZ
Regulatory Status RUO
Purification Method Affinity chromatography
Gene Alias AA408868; FLJ30225; FLJ34463; Ikappa B-zeta variant 3; ikappaBzeta; I-kappa-B-zeta; IkappaB-zeta; IKBZ; ikB-zeta; IL-1 inducible nuclear ankyrin-repeat protein; INAP; Mail; molecule possessing ankyrin repeats induced by lipopolysaccharide; molecule possessing ankyrin-repeats induced by lipopolysaccharide; NF-kappa-B inhibitor zeta; NFKB inhibitor zeta; NFKBIZ; nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, zeta; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta; OTTHUMP00000214340; RGD1310834
Gene NFKBIZ
Product Type Antibody
Gene ID (Entrez) 304005, 64332, 80859
Formulation PBS with 0.09% sodium azide; pH 7.4
Classification Recombinant Monoclonal
Primary or Secondary Primary
Clone LK2NAP
10

Thermo Scientific™ ELISA Carbonate Coating Buffer

For use as a plate coating buffer for Invitrogen Antibody Pairs or when developing a sandwich ELISA

EDGE ENCOMPASS_PREFERRED
11

Thermo Scientific™ ELISA Wash Buffer (30X)

Concentrated wash buffer for efficient use of storage in the lab

EDGE
12

Thermo Scientific™ Pierce™ Diethanolamine Substrate Buffer (5X)

Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.

EDGE
13

Thermo Scientific™ Pierce™ Stable Peroxide Substrate Buffer (10X)

Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.

EDGE
14

Thermo Scientific™ PNPP Substrates

Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.

EDGE
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type Alkaline Phosphatase
Detection Method Colorimetric
15

Thermo Scientific™ QuantaBlu™ Fluorogenic Peroxidase Substrate Kit
GSA_VA

Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.

EDGE