The Chemiluminescent Substrate Solution (HRP Marker) is a specific triggering reagent for peroxidase labels such as horseradish peroxidase (HRP) and can be used in ELISA procedures. This substrate is an enhanced chemiluminescent substrate enables detection at femtogram range of target by oxidizing luminol in the presence of horseradish peroxidase (HRP) and peroxide.This product is provided in two liquid buffer of Solution A (Luminol-Based Solution) and Solution B (Peroxide Solution). When used, mix Solution A and Solution B in equal volume by 1:1, and it gives a rapid light emission.