Life Science Research ELISA Kits
Life Science Research ELISA Kits
Proteintech Group Inc AuthentiKine™ Human IL-10 ELISA Kit
KE00170 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL10 ELISA kit is to be used to detect and quantify protein levels of endogenous IL10. The assay recognizes human IL10. An antibody specific for IL10 has been pre-coated onto the microwells. The IL10 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IL10 is added to detect the captured IL10 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
Proteintech Group Inc AuthentiKine™ Human IFN alpha 2A ELISA Kit
KE00207 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IFN alpha 2A ELISA kit is to be used to detect and quantify protein levels of endogenous IFN alpha 2A. The assay recognizes human IFN alpha 2A. An antibody specific for IFN alpha 2A has been pre-coated onto the microwells. The IFN alpha 2A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IFN alpha 2A is added to detect the captured IFN alpha 2A protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
Proteintech Group Inc AuthentiKine™ Human VEGF ELISA Kit
KE00216 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The VEGF ELISA kit is to be used to detect and quantify protein levels of endogenous VEGF. The assay recognizes human VEGF. An antibody specific for VEGF has been pre-coated onto the microwells. The VEGF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for VEGF is added to detect the captured VEGF protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
Proteintech Group Inc Mouse IL-1ra/IL-1F3 ELISA Kit
KE10052 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-1ra ELISA kit is to be used to detect and quantify protein levels of endogenous IL-1ra. The assay recognizes mouse IL-1ra. An antibody specific for IL-1ra has been pre-coated onto the microwells. The IL-1ra protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL-1ra is added to detect the captured IL-1ra protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
Proteintech Group Inc Human UCH-L1 ELISA Kit
KE00211 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The UCH-L1 ELISA kit is to be used to detect and quantify protein levels of endogenous UCH-L1. The assay recognizes human UCH-L1. An antibody specific for UCH-L1 has been pre-coated onto the microwells. The UCH-L1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for UCH-L1 is added to detect the captured UCH-L1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
Proteintech Group Inc Human TGF-beta1 ELISA 96well
TGF-beta is a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. TGF-beta is produced by a number of cell types including regulatory T cells, fibroblasts, epithelial cells, and endothelial cells. TGF-beta acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. TGF-beta plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. TGF-beta appears to promote late stage progression and metastasis in some cancers.
Proteintech Group Inc Human ENO2 ELISA Kit 96well
ENO2, also named as Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), particularly isoform neuron-specific enolase (NSE), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors. ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part.
Proteintech Group Inc Human GMFB ELISA Kit 96well
GMFB, Glia maturation factor beta, is a 17kd protein which initially identified as a nerve growth factor implicated in nervous system development. GMF-beta protein is specific to the brain where it is expressed in glial cells (mainly astrocytes) and insome neurons. GMFB causes differentiation of brain cells, stimulation of neural regeneration, and inhibition of proliferation of tumor cells. GMFB can be considered as a prognostic predictor for SOC(Serous ovarian carcinoma) patients. This kit is used to quantify GMFB level in vivo.
Proteintech Group Inc Human IL22 ELISA Kit 96well
Interleukin 22 (IL22) is a member of the human type I interferon family, which includes IL10. IL22 has the potential to interact with IL10 because it binds to the IL-10R2c chain with IL22R1 in its receptor complex. Binding can be blocked by the soluble receptor, IL22 binding protein (IL22BP). Although known as a Th17 cytokine, IL22 is also expressed by a wide range of immune cells, including NK T, γδ T, and NK cells. IL22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. IL22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL10.
Proteintech Group Inc Human P21 ELISA Kit 96well
CDKN1A (p21, CIP1, WAF1), a cyclin-dependent kinase inhibitor, is necessary for proper control of the cell cycle and premature senescence. p21 is a cyclin-dependent kinase (CDK) inhibitor that suppresses proliferation by inhibiting CDK2 and CDK1 activity at the G1/S and G2/M transitions. p21 is a major mediator of p53 to induce cell cycle arrest in G1. p21 can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. p21 was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Overexpression of p21 decreases the expression levels of cell cycle progression genes and upregulates senescence-inducing genes.
Proteintech Group Inc Human TNFR1 ELISA Kit 96well
Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling. This receptor can be released to the extracellular space by two mechanisms, ectodomain shedding and constitutive release of full-length 55-kDa TNFR1. Soluble TNFR1 (sTNFR1) could function as TNF-binding protein, inhibiting TNF bioactivity. This ELISA kit can be used to measure natural and recombinant human sTNFR1.
Proteintech Group Inc Human HER2 ELISA Kit 96well
HER2, also known as ErbB2 and Neu, is a member of the epidermal growth factor (EGF) receptor family. Overexpression of HER2 have been reported in numerous cancers, including breast, ovarian, lung, gastric, and oral cancers. It is a therapeutic target for the treatment of breast cancer and other carcinomas. HER2 is a 185-kDa transmembrane glycoprotein, the extracellular domain (ECD) of which can be cleaved from the cell surface by matrix metalloproteases and then released into blood. Elevated serum HER2 levels are reported in 23% to 80% of patients with metastatic breast cancer. Raising serum HER2 levels have been correlated with indicators of poor prognosis and a poor response to chemotherapy.
Proteintech Group Inc Human CCL5 ELISA Kit 96well
CCL5, also known as RANTES, belongs to the CC chemokine family. It is strongly expressed in the human and murine liver upon injury. CCL5 has been found to be produced by numerous cell types, including platelets, macrophages, endothelial, and stellate cells. CCL5 recruits T cells, dendritic cells, eosinophils, NK cells, mast cells, and basophils to sites of inflammation by interacting with 3 specific G protein-coupled receptors: CCR1, CCR3, and CCR5. While CCL5 may promote efficient anti-tumour immune responses, it has also been associated with cancer progression and metastasis.
Proteintech Group Inc Human SERPINE1 ELISA 96well
SERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. It is produced by the vascular endothelium, the liver, the monocytes/macrophagues, the platelets and the adipose tissue. High plasma levels of PAI-1 have been associated with an increased risk of suffering cardiovascular diseases. It is implicated in the pathogenesis of obesity, insulin resistance and type 2 diabetes. In several tumor types, SERPINE1 expression is up-regulated and it has been described as a poor prognostic marker. Besides its prognostic value, SERPINE1 expression has been validated as a marker for therapy decision making in patients with node-negative breast cancer. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia.
Proteintech Group Inc Human CCL3 ELISA Kit 96well
Chemokine (C-C motif) ligand 3 (CCL3), also known as MIP-1α, belongs to the family of chemokines. CCL3 has been found in the central nervous system and its cognate receptors, CCR1 and CCR5, have been reported to be expressed by astrocytes, microglia and neurons. CCL3 and its receptors, CCR1 and CCR5, also contribute to the development of bone disease in multiple myeloma by supporting tumor growth and regulating osteoclast differentiation. CCL3 is also associated with the regulation of cell growth, angiogenesis, and metastasis of different tumors such as melanoma, renal cell carcinoma, and colorectal cancer. Moreover, CCL3 enhances cell migration and metastasis by up-regulating matrix metalloproteinase-2 (MMP)-2 expression in chondrosarcoma cells.