Recombinant Protein;1MG;Human FOLR2, His Tag (FO2-H5223) is expressed from human 293 cells (HEK293). It contains AA Thr 17 - His 228 (Accession # P14207-1).;This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.5 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to N-linked glycosylation which has been verified by PNGase F digestion.;Immobilized Folic acid-BSA conjugate at 5 μg/mL (100 μL/well) can bind Human FOLR2, His Tag (Cat. No. FO2-H5223) with a linear range of 2-20 ng/mL (QC tested).;Immobilized Folic acid-BSA on CM5 Chip can bind Human FOLR2 Protein, His Tag (Cat. No. FO2-H5223) with an affinity constant of 2.14 nM as determined in a SPR assay (Biacore T200) (Routinely tested).