Sartorius Vivaflow™ 200 Crossflow Cassettes

Offers a standard for ease of use, reliability and flexibility unrivalled by other filtration systems. Sartorius Vivaflow 200 Crossflow Cassettes are ready to use, reusable laboratory crossflow cassettes for concentrating up to 5L samples. Available in different MWCOs and membrane types for different application needs.

Catalog Number	Mfr. No.	Material (Membrane)	MWCO
14-558-320	Sartorius VF20P2	PES	30 kDa
14-558-318	Sartorius VF20P0	PES	10 kDa
14-558-322	Sartorius VF20P4	PES	100 kDa
14-558-315	Sartorius VF20H0	Hydrosart	10 kDa
14-558-321	Sartorius VF20P3	PES	50 kDa
14-558-317	Sartorius VF20H2	Hydrosart	30 kDa
14-558-319	Sartorius VF20P1	PES	5 kDa
14-558-323	Sartorius VF20P7	PES	0.2um
14-558-314	Sartorius VF20H4	Hydrosart	100 kDa
14-558-316	Sartorius VF20H1	Hydrosart	5 kDa

Description

Rapid, Efficient Concentration

• Optimally suited for cell culture supernatant concentration, virus concentration and water concentration
• Utilizes a 200cm² PES or Hydrosart membrane for high flow rate
• Thin-channel, flip-flow recirculation path provides high crossflow velocities with minimum pump speed
• Fast concentration using one or two cassettes
  • A single module typically concentrates 1L by a factor of 50 in less than 30 minutes
  • Two modules used in parallel concentrates 5L by a factor of 50 in under 75 minutes
• Near total recovery of the concentrate is achieved with a single rinse
• A 500ml sealed diafiltration reservoir can be added for rapid and convenient buffer exchange

Convenient and Easy to Use

• One unit comes with all necessary accessories (except pump and pump head)
  • Includes tubing, pressure indicator, flow restrictor and high pressure pump tubing
  • Required equipment: peristaltic pump with a size 16 pump head
• Can be easily cleaned and re-used several times
• Clear housing for process monitoring
• Ready-to-use, disposable cassettes
• Easily set up two cassettes to run simultaneously for faster concentration
  • Use a pre-assembled Y connector with the correct tubing sizes and fittings
  • Size 16 tubing is used for connecting the two Vivaflow 200 units
  • Size 15 tubing to connect to the pump, with a size 15 pump head

Specifications

• Material: Acrylic
• For Use With (Application): Cell culture supernatant concentration, virus concentration and water concentration
• Max. Pressure: 60psi
• Quantity: 1 Each
• Temperature (Metric) Max: 60°C
• Volume (Metric): 500 mL to 5 L
• Dimensions (L x W x H): 4.96 x 1.49 x 5.43 in. (12.6 x 3.8 x 13.8cm)
• Width (Metric): 3.8 cm
• Height (Metric): 13.8 cm

• Filtration Area: 200 cm²
• Includes: Pressure indicator, flow restrictor and size 16 PVC peristaltic tubing and fittings
• Pump Flow Rate: 200 to 400mL/min.
• Temperature (English) Max.: 140°F
• Type: Crossflow Cassette
• Volume (Metric) Hold Up: 5.3 mL
• Length (Metric): 12.6 cm
• Width (Metric) Channel: 1 cm
• Height (Metric) Channel: 0.04 cm