Gibco™ Viral Production Cells 2.0

Gibco Viral Production Cells 2.0 are a clonal cell line derived from the HEK293F parental cell line and a core component of the AAV-MAX Helper-Free AAV Production System.

Catalog Number	Mfr. No.	Format
A49784	Gibco™ A49784	1 vial
A51218	Gibco™ A51218	6 vials

Description

• Gibco Viral Production Cells 2.0 are a clonal cell line derived from the HEK293F parental cell line
• They are a core component of the AAV-MAX Helper-Free AAV Production System
• The cells have been adapted to the chemically defined Viral Production Medium and are maintained in suspension culture in that medium
• Viral Production Cells 2.0 are highly transfectable using the AAV-MAX Transfection Kit for adeno-associated virus (AAV) production
• Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 10⁷ viable cells/mL in Viral Production Medium and 10% DMSO
• Cells should be thawed directly into Viral Production Medium supplemented with 4 mM GlutaMAX Supplement (Cat. No. 35050038) for optimal performance

Features of the Viral Production Cells 2.0 include:

• Clonal, 293F-derived, high-producer cell line
• Absence of SV40 large T antigen
• Optimized for high-density, suspension culture (>12 million cells/mL) in chemically defined medium
• Enable production of high AAV titers, greater than 5 x 10¹⁰ vg/mL (unpurified)
• Robust scalability and passage stability

Specifications

• Cell State: Frozen
• Cell Type: Kidney (Embryonic)
• Form: Frozen
• For Use With (Application): AAV Production
• No. of Cells: 1 x 10⁷ cells
• Product Type: Viral Production Cells
• Source: Human

• Cell Line: Viral Production Cells 2.0
• Culture Type: Suspension Cell Culture
• Species: Human
• Media Recommendation: Gibco Viral Production Medium
• Product Line: AAV-MAX
• Quantity: 1 mL

For Research Use Only. Not for use in diagnostic procedures.