VCAM-1 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Pepro Tech Inc 500P300BT1MG

Catalog No. 500P300BT1

Description

AA Seq. of rec. protein: FKIETTPESR YLAQIGDSVS LTCSTTGCES PFFSWRTQID SPLNGKVTNE GTTSTLTMNP VSFGNEHSYL CTATCESRKL EKGIQVEIYSF PKDPEIHLSG PLEAGKPITV KCSVADVYPF DRLEIDLLKG DHLMKSQEFL EDADRKSLET KSLEVTFTPV IEDIGKVLVC RAKLHIDEMD SVPTVRQAVK ELQVYISPKN TVISVNPSTK LQEGGSVTMT CSSEGLPAPE IFWSKKLDNG NLQHLSGNAT LTLIAMRMED SGIYVCEGVN LIGKNRKEVE LIVQEKPFTV EISPGPRIAA QIGDSVMLTC SVMGCESPSF SWRTQIDSPL SGKVRSEGTN STLTLSPVS FENEHSYLCT VTCGHKKLEK GIQVELYSFPR DPEIEMSGGLV NGSSVTVSCK VPSVYPLDRLE IELLKGETILE NIEFLEDTDM KSLENKSLEMT FIPTIEDTGKA LVCQAKLHID DMEFEPKQRQ STQTLYVNVA PRDTTVLVSP SSILEEGSSV NMTCLSQGFP APKILWSRQL PNGELQPLSE NATLTLISTK MEDSGVYLCE GINQAGRSRK EVELIIQVTP KDIKLTAFPS ESVKEGDTVI ISCTCGNVPE TWIILKKKAE TGDTVLKSID GAYTIRKAQL KDAGVYECES KNKVGSQLRS LTLDVQGREN NKDYFSP. Sandwich ELISA: To detect hVCAM-1 by sandwich ELISA (using 100 uL/well antibody solution) a conc. of 0.25-1.0 ug/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Hu VCAM-1 (500-P300) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of rec. hVCAM-1. WB: To detect hVCAM-1 by WB analysis this antibody can be used at a conc. of 0.1-0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for rec. hVCAM-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.

Specifications

• Antigen: VCAM-1
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: VCAM1
• Gene Alias: adhesion molecule VCAM-1; CD106; CD106 antigen; DKFZp779G2333; INCAM100; INCAM-100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; vascular cell adhesion molecule; vascular cell adhesion molecule 1; vascular cell adhesion molecule-1; vascular cell adhesion protein 1; V-CAM 1; Vcam1; Vcam-1; VCAM1B
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 7412
• Content And Storage: -20°C

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P19320
• Gene Symbols: VCAM1
• Immunogen: HEK293 cells-derived, 74.1 kDa Recombinant Human VCAM-1
• Quantity: 2 x 500 μg
• Primary or Secondary: Primary
• Target Species: Human
• Form: Lyophilized