Invitrogen™ TrueCut™ Cas9 Protein v2

A next-generation CRISPR Cas9 protein engineered to deliver maximum editing efficiency.

Supplier:  Invitrogen™ A36499

Catalog No. A36499

Description

TrueCut Cas9 Protein v2 is recombinant Streptococcus pyogenes Cas9 (wt) protein, purified from E. coli, for genome editing with CRISPR technology. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of CRISPR/Cas9 system. Incorporation of nuclear localization signals (NLS) aid delivery to the nucleus, increasing the rate of genomic DNA cleavage.

• Consistently high editing efficiency in all tested cell lines including standard, immune, primary, and stem, with up to 2x higher editing efficiency in difficult targets than the competition
• High quality—manufactured under strict ISO 13485 quality standards
• Validated protocols for large number of cell types to achieve success faster
• Transfection-ready, using either lipid-mediated transfection reagents or electroporation
• Eliminates time-consuming cloning steps
• Available in 2 concentrations: 1μg/μL for use in standard editing scenarios and 5μg/μL for optimization of editing conditions in more difficult scenarios such as in primary or embryonic cells, microinjection, or when screening multiple gRNA sequences at a time

Certifications

CoA

Specifications

• Concentration: 5 μg/μL
• Grade: Molecular Biology
• Accession Number: NC_002737.2:854751-858857
• Gene ID (Entrez): 901176
• Gene Alias: cas5, csn1
• Protein Tag: Untagged
• Validated Application: Genome Editing
• Research Category: Genome Editing

• Components: 100μL TrueCut Cas9 Protein v2 (5mg/mL)
• Content And Storage: 100 µL TrueCut Cas9 Protein v2 (5 mg/mL)
  Store at -5 to -30°C.
  
  
• Expression System: E. coli
• Gene Symbol: Spy_1046
• Protein Form: Recombinant
• Shipping Condition: Dry Ice
• Product Type: Cas9 Protein v2
• Product Line: Invitrogen™