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Edvotek™ Transformation of E. coli with Green and Blue Fluorescent Protein Plasmids Classroom Kit
Helps students develop an understanding of bacterial transformation by plasmid DNA
Supplier: Edvotek™ 222
Alert:
Transformation experiments contain antibiotics which are used for the selection of transformed bacteria. Students who have allergies to antibiotics such as penicilllin, ampicillin, kanamycin or tetracycine should not participate in this experiment. All components intended for educational research only. They are not to be used for diagnostic or drug purposes, nor administered to or consumed by humans or animals.
Includes: instructions, BactoBeads™, plasmid DNA, IPTG, ampicillin, transformation solution, ReadyPour™ agar, Luria broth, petri dishes, sterile pipets and loops
Requires :
- Automatic micropipet (5-50μL) and tips
- 2 water baths (37°C and 42°C)
- Thermometer
- Incubation oven (34°C and 37°C)
- Pipet pumps or bulbs
- Ice
- Marking pens
- Bunsen burner, hot plate or microwave oven
- Hot gloves
- Long-ave UV light (EDVOTEK No. 969 recommended)
Description
- Enables students to observe the acquired phenotypic trait of green or blue fluorescent protein exhibited by transformed bacterial cells
- Transformants acquire green (GFP) or blue (BFP) fluorescent protein genes contained in two different plasmids; the GFP gene was mutated to produce the blue protein
- Transformed cells are selected based on antibiotic resistance
- Colonies which express GFP or BFP protein are visibly green and pale blue and will fluoresce when exposed to long-wave UV light
Order Info
Class size: 10 experiments. Shipped ambient. Supercoiled pFluoroGreen™, Supercoiled pFluoroBlue™, ampicillin, and IPTG require -20°C storage. Note:Transformation cell slant should not be frozen.
Specifications
| Transformation of E. Coli Kit | |
| Instructions, lyphocells, plasmid dna, iptg, ampicillin, transformation solution, readypour™ agar, luria broth, petri dishes, sterile pipets and loops | |
| 10 Lab Groups |
| Helps students develop an understanding of bacterial transformation by plasmid dna | |
| Waterbath , 37°C incubation oven, microwave or hot plate, long wave UV lamp |
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