Invitrogen™ TOPO™ XL-2 Complete PCR Cloning Kit, with One Shot™ OmniMAX™ 2 T1^R Chemically Competent E. coli Cells

Provides all the necessary elements for efficient cloning of extra-long PCR products (up to 13 kb)

Supplier:  Invitrogen™ K805010

Catalog No. K805010

Includes: Box 1:

• pCR XL-2-TOPO vector
• Salt Solution
• T3 Primer
• T7 Primer
• XL Control PCR Template
• XL Control PCR Primers
• Platinum SuperFi Green PCR Master Mix
• SuperFi GC Enhancer
• Nuclease-free water

Box 2:

• Gel Solubilization Buffer (L3)
• Binding Buffer (B2)
• Wash Buffer (W1)
• Elution Buffer (E1)
• PureLink Clean-up Spin Columns (in Wash Tubes)
• PureLink Recovery/Elution Tubes

Box 3:

• One Shot OmniMAX 2 T1R chemically competent E. coli
• S.O.C. Medium
• pUC 19 Control DNA

Description

The kit uses the linearized and topoisomerase 1-activated pCR™-XL-2-TOPO vector, which is compatible with the cloning of blunt-end PCR fragments.  Amplification of long PCR fragments is enabled by Platinum™SuperFi™ Green PCR Master Mix, which is included in the kit.  Topoisomerase I activation of the vector allows PCR products to be ligated in just 5 minutes on your bench–top, resulting in high cloning efficiency (up to 90% positive recombinants with 10 kb fragments).

The pCR-XL-2-TOPO vector includes:

• ccdB gene for positive selection
• EcoR I site flanking the PCR product insertion site for easy excision of inserts
• Ampicillin- and kanamycin-resistance genes for your choice of selection in E. coli
• T7 promoter/priming site for in vitro RNA transcription and sequencing
• T7, T3, and M13 forward and reverse primer sites for sequencing

The TOPO XL-2 Complete PCR Cloning Kit includes:

• TOPO XL-2 PCR Cloning Kits containing the pCR-XL-2-TOPO vector
• Platinum SuperFi Green PCR Master Mix—featuring a proofreading DNA polymerase (>100X fidelity compared to Taq) with high processivity and a density gradient to generate accurate, long PCR amplicons ready to load onto an agarose gel for gel extraction
• PureLink™ Quick Gel Extraction and PCR Purification Combo Kit—designed to purify DNA fragments from agarose gels or direct PCR purification using a silica-based spin cartridge in < 30 minutes
• One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli Cells—an improved chemically competent cell line, perfect for use in all cloning applications.

Specifications

• Product Type: Cloning Kit
• Quantity: 10 Reactions
• Cloning Method: Blunt TOPO
• Content And Storage: Box 1: Store at -5 to -30°C.
  • pCR XL-2-TOPO vector
  • Salt Solution
  • T3 Primer
  • T7 Primer
  • XL Control PCR Template
  • XL Control PCR Primers
  • Platinum SuperFi Green PCR Master Mix
  • SuperFi GC Enhancer
  • Nuclease-free water
  
  Box 2: Store at room temperature.
  • Gel Solubilization Buffer (L3)
  • Binding Buffer (B2)
  • Wash Buffer (W1)
  • Elution Buffer (E1)
  • PureLink Clean-up Spin Columns (in Wash Tubes)
  • PureLink Recovery/Elution Tubes
  
  Box 3:
  • One Shot OmniMAX 2 T1R chemically competent E. coli
  • S.O.C. Medium
  • pUC 19 Control DNA
  
  Store competent cells and pUC19 at -65 to -85°C.
  
  
• Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice
• Vector: TOPO Cloning Vectors
• Cell Type: Chemically Competent E. coli

• For Use With (Application): PCR Cloning
• No. of Reactions: 10
• Format: Kit
• Antibiotic Resistance Bacterial: Ampicillin (AmpR), Kanamycin (KanR)
• Product Line: TOPO™
• Bacterial or Yeast Strain: OmniMAX™2

For Research Use Only. Not for use in diagnostic procedures.