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New England Biolabs, Inc. Thermostable USER III Enzyme - 50 units
Supplier: New England Biolabs, Inc. M5509S
Thermostable USER (Uracil-Specific Excision Reagent) III Enzyme generates a single nucleotide gap at the location of a uracil. Thermostable USER III Enzyme is a mixture of Afu Uracil DNA glycosylase (UDG) and the DNA glycosylase-lyase Endonuclease IV. Afu UDG catalyzes the excision of a uracil base, forming an abasic (apyrimidinic) site while leaving the phosphodiester backbone intact. The lyase activity of Endonuclease IV breaks the phosphodiester backbone at the 3’ and 5’ sides of the abasic site so that base-free deoxyribose is released leaving a 5’ deoxyribose phosphate (dRP) and a 3’-hydroxyl. Thermostable USER III Enzyme also has 3’-diesterase activity which can remove 3’ phosphate, 3’-alpha, beta-unsaturated aldehyde, phosphoglycoaldehyde, and other 3' blocking groups. Thermostable USER III Enzyme is optimally active at 65C.
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