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Cytiva Thermo Sequenase DNA Polymerase
Novel thermostable DNA polymerase that uses dideoxynucleotide triphosphates (ddNTPs) as readily as deoxynucleotide (dNTP) substrates
$1431.00 - $9234.00
Specifications
| No. of Reactions | 32 units/μL |
|---|---|
| Source | E. coli clones that overproduce the protein |
| Catalog Number | Mfr. No. | dimQuantity | Price | Quantity & Availability | |||||
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| Catalog Number | Mfr. No. | dimQuantity | Price | Quantity & Availability | |||||
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09-928-126
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Cytiva
E79000Y |
Each for $1,431.00
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09-928-127
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Cytiva
E79000Z |
Each for $9,234.00
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Description
- Uniform and easy-to-read band patterns
- Better incorporation of ddNTPs helps avoid secondary structures that can cause unreadable sequences
- Read-through of difficult sequences (e.g., GC-rich), due to the ability of thermostable enzymes to function at elevated temperature used for sequencing
- ddNTPs are chain-elongating inhibitors of DNA polymerase, which are used in the Sanger method for DNA sequencing
- Each nucleotide base of this particular type has a probability of being bonded not to a deoxynucleotide but rather a dideoxynucleotide, which ends chain elongation
- An excellent choice for cycle sequencing where template quantity limitations preclude the use of T7 Sequenase DNA polymerase
- Can also be used in many SNP genotyping, genotyping by sequencing, or genome sequencing applications where primer extension is employed
Specifications
| 32 units/μL | |
| E. coli clones that overproduce the protein |
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