Thermo Scientific™ RevertAid RT Reverse Transcription Kit

A complete system for efficient synthesis of first strand cDNA from RNA templates.

Supplier:  Thermo Scientific™ K1691

Catalog No. FERK1691

Description

The kit uses RevertAid Reverse Transcriptase which has lower RNase H activity, compared to AMV reverse transcriptase. The recombinant RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with reverse transcription reaction, as it maintains activity at temperatures up to 55°C. The oligo(dT)₁₈ anneals selectively on the poly(A) tail of mRNA. Random hexamer primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5'-end regions of mRNA or cDNA synthesis using RNA without poly(A) tail, e.g., micro RNAs. Gene-specific primers may also be used with the kits. The first strand of cDNA can be directly used as a template in PCR (see RT-PCR protocol), real-time PCR or in second strand cDNA synthesis (see protocol).

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA templates from degradation.
The RevertAid First Strand cDNA Synthesis Kit is supplied with both oligo(dT)₁₈ and random hexamer primers. The oligo(dT)₁₈ primer anneals selectively to the poly(A) tail of mRNA. Random hexamer primers do not require the presence of the poly(A) tail, therefore, they can be used for transcription of the 5'-end regions of mRNA or cDNA synthesis of RNA species lacking a poly(A) tail (e.g., microRNAs). Gene-specific primers may also be used with the kit.
Highlights
• Full-length first strand cDNA up to 13 kb
• Optimum reaction temperature 42°C
• Complete kit - all the components for the RT reaction are included
Applications
• First strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis
Includes
The RevertAid First Strand cDNA Synthesis Kit contains RevertAid Reverse Transcriptase, RiboLock RNase Inhibitor, 5X Reaction Buffer, dNTP Mix, Random Hexamer Primer, Control GAPDH RNA, 10 μM Forward GAPDH Primer, 10 μM Reverse GAPDH Primer, and nuclease-free water.
The RevertAid RT Kit contains RevertAid Reverse Transcriptase, RiboLock Rnase Inhibitor, 5X Reaction Buffer, dNTP Mix, Random Hexamer Primer, and nuclease-free water.

Hazardous:No
>Quality Control:The kit is functionally tested in RT-PCR using 100 fg control GAPDH RNA and GAPDH control primers generated a 496 bp product visible on agarose gel after ethidium bromide staining.
> Storage Condition:-20°C

First strand cDNA synthesis for RT-PCR and real-time RT-PCR (1, 2); Construction of full length cDNA libraries; Generation of probes for hybridization; aRNA synthesis.

Order Info

Orders placed Monday through Wednesday ship next day; Thursday and Friday orders ship the following Monday.

Specifications

• Content And Storage:

  • RevertAid Reverse Transcriptase
  • Ribolock RNase Inhibitor
  • 5X Reaction Buffer
  • dNTP Mix
  • Random Hexamer Primer
  • Nuclease-free water
  
  Store at –20°C.
  

  
  
• Reaction Speed: 60 min.
• Optimal Reaction Temperature: 42°C
• Ribonuclease H Activity: Yes
• For Use With (Application): Real Time PCR (qPCR)
• No. of Reactions: 500 Reactions
• Reagent Type: Reverse Transcription
• Starting Material: RNA

• Format: Kit
• Technique: Reverse Transcription
• Reverse Transcriptase: RevertAid
• Shipping Condition: Dry Ice
• Final Product Type: First-Strand cDNA
• Reaction Format: Separate components
• Size (Final Product): Up to 13 kb