Thermo Scientific™ Pierce™ Mouse Embryonic Fibroblast Isolation Kit

Easily isolate and culture highly viable mouse embryonic fibroblasts

Supplier:  Thermo Scientific™ 88279

Catalog No. PI88279

Description

The Thermo Scientific Pierce Mouse Embryonic Fibroblast Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of fibroblasts from mouse embryos.

The Mouse Embryonic Fibroblast Isolation Kit has been designed to obtain high viability and purity of embryonic fibroblasts using validated reagents and an optimized procedure. The kit contains a tissue-specific dissociation enzyme and media formulated for primary cell culture that promotes the isolation growth of functional fibroblasts. In addition, MEF isolated and cultured with Pierce MEF Isolation Kit can be cryopreserved for future use with very minimal loss of viability.

Highlights:

• Optimized - procedure and reagents optimized for viability, yield, purity, and ease-of-use
• Yield - provides a 2-fold increase in yield compared to do-it-yourself methods and other commercial kits
• Functional - cultured fibroblasts express the appropriate biochemical markers

Includes:

• Complete kit includes Fibroblast Isolation Enzyme, Hanks' Balanced Salt Solution (HBSS), and DMEM for Primary Cell Isolation

Applications:

• Generation of immortalized cell lines from transgenic mice
• Feeder layer for stem cells
• IHC and IF studies

Product Details:
Because it uses an optimized tissue-specific dissociation enzyme, this kit isolates fibroblasts with greater yield and cell viability than cells isolated by existing methods. Isolated MEF express fibroblast-specific protein markers, confirming their viability and unperturbed metabolic function. After isolation, the mouse fibroblasts may be immortalized by continuous passage for the establishment of unique cell lines. They may also be used as a cell culture model for a diverse range of studies such as gene regulation and stem cell research [1,2,3,4].
General References:
1. Wang, S., et al. (2012) Inhibition of AMPK by doxorubicin accentuates genotoxic stress and cell death in mouse embryonic fibroblasts and cardiomyocytes: role of p53 and SIRT1. J. Biol. Chem. 287(11):8001-12.
2. Blackman, B.E., et al. (2009) PDE4D and PDE4B function in distinct subcellular compartments in mouse embryonic fibroblasts. J. Biol. Chem 286 (14):12590-601.
3. Vierbuchen, T., et al. (2010) Direct conversion of fibroblasts to functional neurons by defined factors. Nature 463:1035-41.
4. Imamura, M., et al. (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 126(4):663-76.

Specifications

• Product Type: Cell Isolation Kit
• Cell Type: Fibroblasts
• Product Line: Pierce™
• Sufficient For: 50 Samples

• Content And Storage: Sufficient For: Isolation of MEFs from 50 embryonic mouse somatic tissues
  • DMEM for Primary Cell Isolation, 500 mL
  • MEF Isolation Enzyme (with papain), 5 vials
  • Hanks' Balanced Salt Solution (HBSS without Ca²⁺, Mg²⁺), 500 mL
  
  Store kit at 4°C.
• Target Species: Mouse
• For Use With (Application): Stem dells, immunohistochemistry and immunofluorescence
• Quantity: 1 Kit

For Research Use Only. Not for use in diagnostic procedures.