Thermo Scientific™ B-PER™ Complete Bacterial Protein Extraction Reagent

Efficient, gentle lysis and extraction of E.coli and other bacterial cells

Includes: Reagent packages are available in Tris and phosphate buffer formulations; Kits contain Tris-based B-PER Reagent plus lysozyme and DNase I enzymes.

Catalog Number	Mfr. No.	Quantity
PI89821	Thermo Scientific™ 89821	250 mL
PI89822	Thermo Scientific™ 89822	500 mL

Description

• An easy-to-use cell lysis reagent that effectively disrupts cells and solubilizes native or recombinant proteins without denaturation
• All B-PER Reagents are compatible with downstream applications such as affinity chromatography (e.g., immobilized metal affinity chromatography, glutathione chromatography), SDS-PAGE, and protein quantification (e.g., Pierce BCA Protein Assay, Pierce 660nm Protein Assay)
• This all-inclusive formulation combines the lysis reagent with lysozyme, and a universal nuclease to enable mild extraction from both gram-negative and gram-positive bacteria
• Depending on the particular application protease inhibitors, salts, reducing agents, denaturants, and chelating agents may be added to the reagent.

Features of the B-PER Bacterial Protein Extraction Reagent:

• Detergent in Tris buffer with Lysozyme and Universal Nuclease
• Improved cell lysis and DNA digestion, thereby releasing soluble proteins and reducing viscosity to increase yields
• Recovery of large molecular weight proteins (high MW proteins)
• Cost-effective equivalent to Merck Millipore Bugbuster™ Master Mix
• More effective than traditional sonication and typical homemade lysis buffers, many of which include detergents and components that interfere with downstream applications

Specifications

• Content And Storage: Store at 4°C.
• Product Line: B-PER™
• Formulation: Proprietary detergent in 20mM Tris buffer, pH 7.5, containing lysozyme and universal nuclease
• Product Type: Bacterial Protein Extraction Reagent

For Research Use Only. Not for use in diagnostic procedures.