Thermo Scientific™ MuSeek Library Preparation Kit, Illumina compatible

Illumina-compatible next-gen sequencing (NGS) libraries from as little as 50ng of starting DNA. DNA fragmentation and transposon-tagging are accomplished in one step.

Supplier:  Thermo Scientific™ K1361

Catalog No. FERK1361

Description

Thermo Scientific MuSeek Library Preparation Kit, Illumina compatible is designed for generation of high-quality genomic DNA libraries for sequencing with the Illumina™ MiSeq™ and HiSeq™ systems. The fast protocol utilizes the MuA transposase enzyme, which catalyzes simultaneous fragmentation of double-stranded target DNA and tagging the fragment ends with transposon DNA. In a subsequent PCR step the platform-specific adapters are added using a robust and accurate Thermo Scientific™ Phusion™ High-Fidelity DNA polymerase. Starting with only 50ng or 100ng of sample the protocol can be used to generate 100 bp to 1,000 bp insert libraries. The kit contains components sufficient for 12 fragmentation reactions and the subsequent adapter-addition polymerase chain reactions. Sequencing primers required for sequencing libraries prepared by this method are provided as well.

Highlights

• Convenient – no physical shearing, end-repair and ligation steps
• Fast – generates NGS fragment libraries in less than 90 minutes
• Low sample input – DNA fragment libraries from 50ng DNA input
• Indexing compatible – MuSeek Index Set 1 enables preparation of up to 24 dual-indexed DNA libraries for sequencing in one run

Applications

• DNA fragment library preparation for downstream use in next generation sequencing workflow
• Library preparation from gDNA, ds DNA, bacterial DNA, DNA amplicons

Includes

• MuSeek Enzyme Mix, IL
• MuSeek Fragmentation Reaction Buffer
• MuSeek Stop Solution
• Water, nuclease-free
• Control DNA
• MuSeek Adapter Addition Primer Mix, IL
• MuSeek Adapter Addition Reaction Buffer, IL
• Phusion™ Hot Start II High-Fidelity DNA Polymerase, IL
• MuSeek Sequencing Primer, Read 1
• MuSeek Sequencing Primer, Read 2
• MuSeek Sequencing Primer, Index Read

Notes
The kit is shipped on dry ice. Upon arrival store at -20°C, avoid repeated freeze-thaw cycles. The Stop Solution can be stored at room temperature.

Specifications

• Product Type: Library Preparation Kit
• For Use With (Equipment): Illumina™ HiSeq 2500, Illumina ™ MiSeq
• Sequencing Type: Genome & DNA Sequencing
• Product Line: MuSeek
• Quantity: 12 Reactions

• Libraries: Fragment Library
• Sample Type: DNA
• Workflow Step: Library Generation
• For Use With (Application): NGS library preparation

For Research Use Only. Not for use in diagnostic procedures.