Thermo Scientific™ SuperSignal™ Molecular Weight Protein Ladder

Ready-to-use mix of recombinant proteins (20 to 150K) with IgG-binding sites for chemiluminescent, fluorescent, chromogenic or other detection methods.

Supplier:  Thermo Scientific™ 84785

Catalog No. PI84785

Description

Thermo Scientific™ SuperSignal Molecular Weight Protein Ladders (20 to 150KDa) are a ready-to-use mix of recombinant proteins with IgG-binding sites that provide reliable and proportional band intensities in stained gels and immunoblots developed with chemiluminescent, fluorescent, chromogenic or other detection systems.

The SuperSignal Molecular Weight Protein Ladders and SuperSignal Enhanced Molecular Weight Protein Ladders contain a ready-to-use stabilized mixture of eight recombinant proteins ranging in size from 20 to 150kDa. These recombinant proteins bind antibodies used in the Western blot through an IgG binding site. The protein markers can then be visualized either using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies. The molecular weight marker mix also contains a special pink tracking dye to monitor the electrophoresis run and membrane transfer without the need for additional colored markers. The standards are compatible with nitrocellulose and PVDF membranes, multiple blocking buffers and many other detection methods.

Two versions of the standard are offered. The regular formulation is appropriate for use with most rabbit and other non-mouse polyclonal antibodies. The enhanced standard is formulated specifically for applications requiring mouse monoclonal antibodies and experiments where very dilute antibody concentrations are used.

Highlights:

• Ready-to-use – liquid format is ready to load and requires no boiling

• Direct visualization – see marker bands with gel stains and blotting detection reagents

• Consistent – even band intensity regardless of detection method

• Compatible – enhanced version for compatibility with mouse monoclonal primary antibodies

• Simple marker sizes – band molecular weights are divisible by 10K, making them easy to remember

• Stable – store long term at -20°C, three months at 4°C or one month at ambient temperature

Additional Features:

• The molecular masses included in these standards are 20, 30, 40, 50, 60, 80, 100 and 150kDa.

Specifications

• Content And Storage: Contents: one vial of 250 μL
  
  Storage buffer: 62.5 mM Tris-H₃PO₄ (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN₃ and 33% glycerol
  
  Storage: Upon receipt store at -20°C for long term storage, or three months at 4°C or one month at ambient
  
  
• Number of Markers: 8
• Ready to Load: Yes
• Stain Type: Unstained
• Molecular Weight (g/mol): 150, 100, 80, 60, 50, 40, 30, 20 kDa
• Quantity: 250 μL
• System Type: Western Blotting, SDS-PAGE

• Detection Method: User defined detection system
• Product Type: Protein Ladder
• Size Range: 20 to 150 kDa
• Gel Compatibility: Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
• Product Line: SuperSignal™
• Shipping Condition: Wet Ice

For Research Use Only. Not for use in diagnostic procedures.