Invitrogen™ SuperScript™ IV VILO™ Master Mix

SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.

Supplier:  Invitrogen™ 11756050

Catalog No. 11-756-050

Description

SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications. The inclusion of ezDNase enzyme further accelerates the RT-qPCR workflow through an extremely simplified genomic DNA removal step.

SuperScript IV VILO Master Mix features include:
• Fast RT reactions in just 10 minutes
• Convenient one-tube cDNA reaction master mix for two-step RT-qPCR
• High yields reduce Ct values by more than two cycles ahead of all other reverse transcription reagents
• Efficient reaction even with low template amounts and suboptimal purity samples
• gDNA removal in as few as two minutes (SSIV VILO Master Mix with ezDNase only)

SSIV VILO Master Mix elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript IV Reverse Transcriptase and further optimized buffer. These components enable efficient cDNA synthesis at higher temperatures and in less time. SSIV VILO master mix provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates. It is your new tool for more efficient and reproducible RT-qPCR.

SuperScript IV VILO Master Mix with ezDNase is available for better removal of genomic DNA and to further accelerate the RT-qPCR workflow. The extremely simplified genomic DNA removal step dramatically reduces the time of the entire reverse transcription protocol and reduces possible variation in gene expression due to RNA loss or damage during conventional DNase treatment.

SSIV VILO master mix results in more cDNA in less time with less pipetting, less variation, less reaction inhibition, less gDNA interference, and with less sample than other cDNA synthesis kits or master mixes for RT-qPCR. Additional ezDNase may also be purchased separately.

Source
Purified from E. coli expressing the pol gene of M-MLV, modified to increase thermostability and half-life, processivity, inhibitor resistance, and to reduce RNase H activity

Performance and quality testing
Assayed for endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease, as well as yield and length of cDNA product

Specifications

• Content And Storage:

  • SuperScript IV VILO Master Mix (200 μL)
  • SuperScript IV VILO Master Mix 'No RT' Control (200 μL)
  • Nuclease-free water (1.25 mL)
  
  Store at –30°C to –5°C.
  
  

• Format: Tube
• PCR Method: 2-step RT-qPCR
• Technique: Reverse Transcription
• Reverse Transcriptase: SuperScript IV
• Shipping Condition: Dry Ice
• Concentration: 5X
• No. of Reactions: 50 Reactions
• Reagent Type: Reverse Transcription
• Starting Material: RNA

• Detection Method: qPCR
• GC-Rich PCR Performance: High
• Reaction Speed: 10 min.
• Optimal Reaction Temperature: 50°C
• Ribonuclease H Activity: Reduced
• For Use With (Application): RT-PCR
• Final Product Type: First-Strand cDNA
• Reaction Format: Master Mix
• Size (Final Product): Up to 10 kb
• Volume: 200 μL

For Research Use Only. Not for use in diagnostic procedures.