Thermo Scientific™ SMART Digest™ Trypsin Kits

Obtain fast, simple, and highly reproducible trypsin protein digestion for peptide characterization and quantitation in biopharmaceutical protein research.

Supplier:  Thermo Scientific™ 60109103B

Catalog No. 03-252-205

Description

Obtain high quality analytical results from protein digests using Thermo Scientific™ SMART Digest™ Kits. A significant advance in sample preparation for biopharmaceutical protein research, the kits provide fast and simple protein digestion with high reproducibility, high sensitivity, and high levels of data quality in a format that's compatible with automation. The SMART Digest Kits are a significant improvement over current in-solution protein digestion technologies, which are not reproducible, have poor sensitivity, and employ protracted methodologies not amenable to automation.

Trypsin Protein Digestion

SMART Digest kits enable you to characterize and quantitate proteins faster, easier, and quicker than ever before. This version of the kit uses trypsin, which cleaves peptides on the C-terminal side of lysine and arginine amino acid residues.

Easy and simple

• Significantly increased speed of sample processing
• Simple process
• Easily automate entire protocol for high-throughput processing

Reliable Results

• Increased reproducibility over existing protocols
• Higher sensitivity
• Higher data confidence

Significantly Reduces Sample Preparation Time

Typically it takes less than 60 minutes (dependent on sample complexity) to achieve full digestion, whereas an in-solution digest can take up to 24 hours.

Cleaner Samples

Reduction in the number of chemicals used and the immobilized trypsin design helps mitigate against chemically induced post translational modifications (PTMs) and autolysis. This results in results which are easier to interpret.

Couple with SOLAμ SPE Plates for Desalting

When used with the SMART Digest kits for desalting, Thermo Scientific™ SOLAμ™ SPE solid phase extraction plates provide a fast, easy-to-use, robust, and easily automatable sample preparation solution for analysis of biomolecules.

Specifications

• Description: SMART Digest Trypsin Kit, Bulk Resin option with SOLAμ/collection plate
• Cleavage: C-terminal side of lysine and arginine amino acids
• Format: SMART Digest Resin, Bulk Non-magnetic
• Product Line: SMART Digest
• Includes: Bulk Non-magnetic SMART Digest Resin, SMART Digest Buffer, Collection Plate, SOLAμ SPE Plate

• Capture: None
• Enzyme: Trypsin
• For Use With (Application): Peptide Quantitation, Peptide Mapping
• Type: Protein Digestion