Thermo Scientific™ SMART Digest™ RNase Kits

Use SMART Digest™ RNase Kits in your oligonucleotide mapping and quantitation workflows to obtain high quality analytical results.

Catalog Number	Mfr. No.	Cleavage	Enzyme
01-257-521	Thermo Scientific™ 60120101	Degrades single-stranded RNA at G residues	RNase T1
01-257-522	Thermo Scientific™ 60120102	Degrades single-stranded RNA at C and U residues	RNase A

Description

Obtain high-quality analytical results from mRNA digestions using Thermo Scientific™ SMART Digest™ RNase Kits. A significant advance in sample preparation for oligonucleotide mapping and quantitation, these kits provide fast and simple mRNA digestion with high reproducibility, high sensitivity, and high levels of data quality in a format that is compatible with automation.

These RNase kits use optimized, heat stable enzymes on magnetic beads, allowing for control of partial digestion to produce required fragments of ideal length for sequencing by HRAM-MS.

Easy and Simple

• A simple process and control of digestion time ensure required fragment length for comprehensive mRNA sequencing by HRAM-MS
• Easily automate the entire protocol for high-throughput processing
• Single nuclease digestion allows full characterization of mRNA

Reliable Results

• Increased reproducibility over existing protocols
• Higher data confidence
• No nuclease contamination of the analytical column

RNase kits are available with with two endoribonuclease enzyme specificities:

• RNase T1 degrades single-stranded RNA at G residues
• RNase A degrades single-stranded RNA at C and U residues

Specifications

• Format: Bulk Magnetic SMART Digest Resin
• Product Line: SMART Digest
• Includes: Bulk Magnetic SMART Digest Resin, SMART Digest Buffer

• For Use With (Application): Oligonucleotide Mapping
• Type: RNA Digestion