Thermo Scientific™ SMART Digest™ Proteinase K Kits

Catalog Number	Mfr. No.	Description
03-252-212	Thermo Scientific™ 60109107	SMART Digest Proteinase K Kit, with Collection plate
03-252-213	Thermo Scientific™ 60109107B	SMART Digest Proteinase K Kit, Bulk Resin option
13-622-454	Thermo Scientific™ 60109107MB	SMART Digest Proteinase K Kit, Magnetic Bulk Resin option
03-252-214	Thermo Scientific™ 60109108	SMART Digest Proteinase K Kit, with Filter/Collection plate
03-252-215	Thermo Scientific™ 60109108B	SMART Digest Proteinase K Kit, Bulk Resin option with Filter/Collection plate
13-622-455	Thermo Scientific™ 60109108MB	SMART Digest Proteinase K Kit, Magnetic Bulk Resin option with Filter/Collection plate
03-252-216	Thermo Scientific™ 60109109	SMART Digest Proteinase K Kit with SOLAμ/collection plate
03-252-217	Thermo Scientific™ 60109109B	SMART Digest Proteinase K Kit Bulk Resin option with SOLAμ/collection plate
03-252-218	Thermo Scientific™ 60109109MB	SMART Digest Proteinase K Kit Magnetic Bulk Resin option with SOLAμ/collection plate

Description

Obtain high quality analytical results from protein digests by using Thermo Scientific™ SMART Digest™ Proteinase K Kits. A significant advance in sample preparation for biopharmaceutical research, the kits provide fast and simple protein digestion with high reproducibility, high sensitivity, and high levels of data quality in a format that's compatible with automation. SMART Digest Kits are a significant improvement over current in-solution protein digestion technologies, which are not reproducible, have poor sensitivity, and employ protracted methodologies not amenable to automation.

Proteinase K Protein Digestion

SMART Digest kits enable you to characterize and quantitate proteins faster, easier, and quicker than ever before. This version of the kit uses proteinase K, which cleaves the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups.

Easy and Simple

• Significantly increased speed of sample processing
• Simple process
• Easily automate entire protocol for high-throughput processing

Reliable Results

• Increased reproducibility over existing protocols
• Higher sensitivity
• Higher data confidence

Significantly Reduces Sample Preparation Time

Typically it takes less than 60 minutes (dependent on sample complexity) to achieve full digestion, whereas an in-solution digest can take up to 24 hours.

Cleaner Samples

Reduction in the number of chemicals used and the immobilized proteinase K design helps mitigate against chemically induced post translational modifications (PTMs) and autolysis. The results are easier to interpret.

Couple with SOLAμ SPE Plates for Desalting

When used with the SMART Digest for desalting, Thermo Scientific™ SOLAμ™ SPE solid phase extraction plates provide a fast, easy-to-use, robust, and easily automatable sample preparation solution for analysis of biomolecules.

Specifications

• Capture: None
• Enzyme: Proteinase K
• Product Line: SMART Digest

• Cleavage: Peptide bond adjacent to carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups
• For Use With (Application): Peptide Quantitation, Peptide Mapping
• Type: Protein Digestion