Thermo Scientific™ SMART Digest™ Immunoaffinity (IA) Protein G Kits

Catalog Number	Mfr. No.	Description
13-622-464	Thermo Scientific™ 60112101	SMART Digest IA Kit, Protein G non-magnetic
13-622-465	Thermo Scientific™ 60112102	SMART Digest IA Kit, Protein G non-magnetic with SOLAμ SPE and collection plate
13-622-466	Thermo Scientific™ 60112103	SMART Digest IA Kit, Protein G magnetic with SOLAμ SPE and collection plate
03-251-710	Thermo Scientific™ 60112104	SMART Digest IA Kit, Protein G magnetic

Description

Obtain fast, simple, highly reproducible, and sensitive immunocapture and protein digestion in a single well using Thermo Scientific™ SMART Digest™ ImmunoAffinity (IA) Kits. These kits were designed to remove the challenges often associated the process of biomarker quantitation by providing a simple and easy capture-digestion workflow that takes only hours instead of overnight. The SMART Digest IA Protein G Kits provide targeted purification of immunoglobulins by utilizing the strong affinity for binding to the Fab and Fc portions of the molecule.

Solving the Challenges of Low-level Quantitation in Complex Matrices

The need to identify low-level biomarkers is forcing the analytical community to deal with extremely high levels of analyte and sample complexity, which makes quantitation challenging.

As these proteins are often present at low levels in complex biological matrices, an immunoaffinity capture step is typically employed to purify the sample and increase sensitivity. This IA step is often followed by protein digestion. Current processes are often time-consuming, multistep, and prone to irreproducibility. SMART Digest IA kits solve these problems.

Immunocapture and Protein Digestion in a Single Well

These IA kits deliver a process that is:

• Fast
• Simple
• Highly reproducible
• Sensitive

Unique Design Reduces Workflow to Hours

In SMART Digest IA Kits, the immunocapture reagents and the temperature-activated, thermally stable trypsin are co-immobilized onto a resin. Following enrichment, the enzyme is activated at elevated temperatures for accelerated digestion under protein denaturing conditions, resulting in a workflow which takes hours rather than overnight.

Specifications

• Capture: Protein G
• Enzyme: Trypsin
• Product Line: SMART Digest

• Cleavage: C-terminal side of lysine and arginine amino acids
• For Use With (Application): Peptide Quantitation
• Type: Immunocapture + Protein Digestion combined on a single bead