Thermo Scientific™ Slide-A-Lyzer™ Dialysis Cassettes, 2K MWCO

Remove salts and small contaminants to maximize sample purity and recovery with Slide-A-Lyzer 2K MWCO dialysis cassettes.

Catalog Number	Mfr. No.	Sample Volume (Metric)
PI66205	Thermo Scientific™ 66205	0.5 mL
PI66203	Thermo Scientific™ 66203	3 mL
PI66212	Thermo Scientific™ 66212	12 mL
PI66230	Thermo Scientific™ 66230	30 mL

Description

Slide-A-Lyzer Dialysis Cassettes (2K MWCO) facilitate the simple and effective removal of salts and small contaminants from proteins and other macromolecules greater than 2,000 Daltons. They are available in four sizes to accommodate sample volumes between 0.5 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing faster buffer exchange and complete sample recovery.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. Slide-A-Lyzer cassettes can be used for a wide range of applications including low-molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers. No knots, clips, or caps are needed to seal the units and prevent leaking and sample loss during the dialysis procedure.

Features of Slide-A-Lyzer Dialysis Cassettes:
• Color-coded—blue frame specifies cassettes with the 2,000-MW pore-size membrane
• Four cassette sizes—select the cassette that best suits your sample volume (0.5 to 30 mL)
• Fast dialysis—flat cassette chamber with two membranes provides high surface area-to-volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
• High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports

Membrane specifications:
• Composition: regenerated cellulose synthesized by the viscose method
• Thickness: 0.9 to 1.2 mil (22.5 to 30 μm)
• Hydration time required before use: 2 minutes for normal volumes or 4 minutes for minimal volumes
• Glycerol content: none
• Sulfur content: 0.169%
• Heavy metals content: trace

Volume ranges:
• 0.5 mL = 0.1 to 0.5 mL
• 3 mL = 0.5 to 3 mL
• 12 mL = 3 to 12 mL
• 30 mL = 12 to 30 mL

Specifications

• Content And Storage: Store at room temperature
• Membrane: Cellulose Membrane, Dialysis
• MWCO: 2.0 kDa

• Purification Target: Buffer Exchange, Protein
• Format: Cassette
• Product Line: Slide-A-Lyzer™