Thermo Scientific Chemicals Safranine O

Description

It is a redox and adsorption indicator. Employed as a biological stain used in histology, cytology, bacteriology. It is commonly used as a counter stain for gram-negative bacteria in the Gram stain procedure. It has been observed to bind to DNA at a pH 2.21. In the Flemming triple stain procedure it is used to stain for chromosomes. In late Paleozoic pteridosperm cuticles it has shown increased contrast, emphasized cutinization differences, and enhanced the three-dimensional morphology of complex epidermal features when compared with other dyes. The compound has also been used with alcian blue in the histochemical study of the distribution and morphometric characteristics of mast cells. It has also been used to detect mucin and cartilage.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
It is a redox and adsorption indicator. Employed as a biological stain used in histology, cytology, bacteriology. It is commonly used as a counter stain for gram-negative bacteria in the Gram stain procedure. It has been observed to bind to DNA at a pH 2.21. In the Flemming triple stain procedure it is used to stain for chromosomes. In late Paleozoic pteridosperm cuticles it has shown increased contrast, emphasized cutinization differences, and enhanced the three-dimensional morphology of complex epidermal features when compared with other dyes. The compound has also been used with alcian blue in the histochemical study of the distribution and morphometric characteristics of mast cells. It has also been used to detect mucin and cartilage.

Solubility
Soluble in water at 20°C, 50g/L. Soluble in alcohol.

Notes
Store away from oxidizing agents. Keep the container tightly closed and place it in a cool, dry and well ventilated condition.

Specifications

Chemical Identifiers

• CAS: 477-73-6
• Molecular Formula: C20H19ClN4
• Molecular Weight (g/mol): 350.85
• MDL Number: MFCD00011759
• InChI Key: QRYAEWIQIBAZOJ-UHFFFAOYSA-N
• Synonym: basic red 2, safranine o, gossypimine, safranin, safranine t, safranin o, safranin t, safranine, tolusafranine, hidaco safranine
• PubChem CID: 2723800
• IUPAC Name: 3,7-dimethyl-10-phenylphenazin-10-ium-2,8-diamine;chloride
• SMILES: [Cl-].CC1=C(N)C=C2C(=C1)N=C1C(C)=C(N)C=CC1=[N+]2C1=CC=CC=C1

• Odor: Odorless
• Quantity: 50 g
• Beilstein: 3924099
• Solubility Information: Soluble in water at 20°C,50g/L. Soluble in alcohol.
• Formula Weight: 350.85
• Chemical Name or Material: Safranine O

Safety and Handling

Hazard Category	H315-H318
Hazard Statement	GHS H Statement H315-H319-H335 Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.
Precautionary Statement	P264b-P280-P302+P352-P305+P351+P338-P310-P332+P313-P362
EINECSNumber	207-518-8
RTECSNumber	SG1623000
TSCA	Yes
Recommended Storage	Ambient temperatures

SDS

RUO – Research Use Only