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Invitrogen™ ReadyProbes™ 2.5% Normal Horse Serum (1X)
SDP

Ready-to-use non-specific blocker for use in immunohistochemistry (IHC), immunocytochemistry (ICC), and immunofluorescence (IF) experiments

Supplier:  Invitrogen™ R37625

Catalog No. R37625


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Description

Description

  • ReadyProbes 2.5% Normal Horse Serum is a ready-to-use non-specific blocker for use in immunohistochemistry (IHC), immunocytochemistry (ICC), and immunofluorescence (IF) experiments, usually when a horse or donkey secondary antibody is used.
  • Incubating cells/tissue with diluted serum from the same or similar species as the host of the secondary antibody typically reduces non-specific binding.
  • Simply cover the tissue section or cells with a few drops of ReadyProbes 2.5% Normal Horse Serum and incubate for 30–60 minutes at room temperature.
  • Ready Probes 2.5% Normal Horse Serum can also be used to dilute primary and secondary antibodies.
  • Ready Probes 2.5% Normal Horse Serum is provided as a ready-to-use reagent with no further dilution or preparation required.
  • The serum is collected from healthy adult animals and the heat-treated, centrifuged, and filtered. It is then checked for cross reactivity using an appropriate immunoassay.
  • The serum is diluted to 2.5% (v/v) in buffer, with 0.08% sodium azide added as a preservative.
Specifications

Specifications

Provided as 50-mL solution at ready-to-use (1X) concentration in a clear plastic dropper bottle.

Store at 2–8°C.

When stored as directed, product can be used for 6 months after receipt.

Liquid
50 mL
Blocking Reagent
Horse
Horse Serum
ReadyProbes™
Room Temperature or Wet Ice
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For Research Use Only. Not for use in diagnostic procedures.