Invitrogen™ PureLink™ Expi Endotoxin-Free Maxi Plasmid Purification Kit

Supplier:  Invitrogen™ A31231

Catalog No. A31231

Description

Purify up to 1.5mg of high quality plasmid DNA that is suitable for advanced transfection applications such as transfection of sensitive cells lines. The PureLink™ Expi Endotoxin-Free Maxi Plasmid Purification Kit utilizes a novel vacuum-assisted purification workflow that dramatically reduces purification to 90 minutes.

• Simple and super-fast protocol - Unlike other anion-exchange-based endotoxin free plasmid purification systems, the PureLink™ Expi Endotoxin-Free Maxi Plasmid Purification Kit utilizes vacuum filtration for lysate clarification and plasmid DNA purification. There is no waiting for gravity-flow columns and typical purification takes 90 minutes.
• High yield - Isolate up to 1.5mg of high quality, endotoxin-free plasmid DNA
• Endotoxin-Free - Purify endotoxin-free (<0.1 EU/ µg) plasmid that is ideal for sensitive applications such as transfection of primary cells, genome engineering, and research on gene therapy or plasmid vaccines.
• Purity - Ultralow levels of protein, RNA and genomic DNA contamination
• Compatibility with ExpiCHOTM and Expi293TM Expression Systems - Plasmid purified using the PureLink™ Expi Endotoxin-Free Plasmid Purification Kit is compatible with the GibcoTM ExpiCHOTM and Expi293TM transient mammalian expression systems.

Specifications

• No. of Reactions: 25 Prep
• Format: Spin Column
• High-throughput Compatibility: Manual Protocols
• Shipping Condition: Room Temperature
• Purification Target: Plasmid DNA
• Purification Time: 90 min
• Scale: Maxi

• For Use With (Application): Transfection
• Content And Storage: • Resuspension buffer
  • Lysis indicator
  • RNase A
  • Lysis buffer
  • Precipitation buffer
  • Endotoxin removal buffer
  • Wash buffer
  • Elution buffer
  • TE buffer
  • Expi Maxi filtration column
  • Expi Maxi DNA binding column
  • 50 mL conical tubes
  
  Endotoxin-free components: elution buffer, water, plasmid storage tubes
  
  
• Prep Scale: > 1 mg (Large-Scale) Plasmid DNA, > 200 µg (Large-Scale) Plasmid DNA
• Product Line: PureLink™
• Sample Type: Bacteria
• Isolation Technology: Anion Exchange Resin