Promega GoScript™ Reverse Transcription System

GoScript Reverse Transcriptase utilizes M-MLV and state-of-the-art buffer technology designed for qPCR to deliver robust, reliable cDNA synthesis of a full range of rare and abundant transcripts, even in the presence of inhibitors.

Supplier:  Promega A5000

Catalog No. PRA5000

Includes: GoScript Reverse Transcriptase, GoScript 5X Reaction Buffer, MgCl₂, Oligo(dT)₁₅ Primer, PCR Nucleotide Mix, Random Primers, Recombinant RNasin Ribonuclease Inhibitor, Nuclease-free water

Description

• Reliable cDNA Synthesis for Sensitive Detection of Expression
• Available as a standalone enzyme, a complete reverse transcription kit or as a master mix with Oligo(dT) or Random Primers. RNasin(R) RNase Inhibitor protection included with Kit and Mixes
• Sensitive transcription of both high-copy and low-copy messages
• Transcribes short and long transcripts; processes through secondary structure

Specifications

• Content And Storage: • MgCl₂, 1 x 750 μL
  • GoScript™ 5X Reaction Buffer, 1 x 300 μL
  • GoScript™ Reverse Transcriptase, 1 x 50 μL
  • oligo(dT)15 primer, 1 x 50 μg
  • Nuclease-free Water, 1 x 200μL
  
  Store at –30° to –10°C.
• For Use With (Application): RT-PCR, Real Time PCR (qPCR)
• No. of Reactions: 50 Reactions

• U.S. Patent 6,242,235, Australian Patent 761757, Canadian Patent 2,335,153 and other patents and patents pending.
• U.S. Patent 5,552,302, Australian Patent 646803 and other patents.