Molecular Probes™ ProLong™ Live Antifade Reagent, for live cell imaging

Prolong Live Antifade Reagent helps prevent the loss of fluorescent signal (photobleaching) during live cell imaging.

Supplier:  Molecular Probes™ P36974

Catalog No. P36974

Description

ProLong Live Antifade Reagent helps prevent the loss of fluorescent signal due to photobleaching during live cell imaging through use of enzymes from the plasma membrane of E. coli. These enzymes metabolize the elements that can cause photobleaching. When dyes are illuminated, they can degrade which reduces the sample’s fluorescent intensity and leads to the creation of free radical singlet oxygen which degrades neighboring dye molecules. ProLong Live Antifade Reagent metabolizes this element, increasing the signal intensity and duration while keeping the background signal low. ProLong Live reagent has little-to-no effect on cellular viability, proliferation, or other cellular functions in experiments up to 48 hours. ProLong Live reagent provides photobleach protection in live cell experiments for fluorophores such as GFP, RFP, Hoechst 33342, MitoTracker™, LysoTracker™, and CellTracker™dyes. ProLong Live Antifade Reagent can be added to any cell culture media or buffer that is suitable for fluorescent imaging.

Specifications

• Quantity: 5 Vials
• Content And Storage: Provided in 5 vials, with 1 mL each. Enough for 5000 assays (0.1 mL imaging solution/assay), or 1000 assays (0.5 mL imaging solution/assay). When stored at ≤-20°C product is stable for at least 6 months, with up to 4 freeze-thaw cycles. When stored at 2–8°C product should be used within 30 days (a precipitant may form at this temperature).
• Product Line: ProLong™
• Volume (Metric): 1 mL

• Product Type: Live Antifade Reagent
• Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice
• Reagent Type: Cell Imaging Reagent
• Description: ProLong™ Live Antifade Reagent for live cell imaging, 5 x 1 mL