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Invitrogen™ PrimeFlow™microRNA Pretreatment Buffer
SDP

Designed to be used in combination with the PrimeFlow™ RNA Assay

Supplier:  Invitrogen™ 8818006

Catalog No. 88-180-06


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Description

Description

  • This PrimeFlow™ microRNA Pretreatment Buffer is designed to be used in combination with the PrimeFlow™ RNA Assay (cat. no. 88-18005-204) for the optimal detection of microRNA and other small RNA by flow cytometry.
  • Because microRNA molecules have a limited number of target probe binding sites per molecule, we recommend using Type 1, Alexa Fluor™ 647 or Type 10, Alexa Fluor™ 568 Target Probe sets for optimal sensitivity.
  • This buffer may also be used in combination with the PrimeFlow™ Fixation/Permeabilization Buffer Set (cat. no. 88-17000-210) to evaluate antibody performance for intracellular targets.
  • Follow the protocol that is attached to this data sheet. Please note that additional changes in antibody performance may be observed after the hybridization steps of the PrimeFlow RNA Assay.
  • The PrimeFlow™ RNA Assay and Target Probe Sets for genes of interest are sold separately.
Specifications

Specifications

PrimeFlow™ microRNA Pretreatment Concentrate (4X): cat. 00-16009, 25 mL, store at 2-8°C
PrimeFlow™ microRNA Pretreatment Diluent: cat. 00-16008, 75 mL, store at 2-8°C
microRNA Pretreatment Buffer
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For Research Use Only. Not for use in diagnostic procedures.