Invitrogen™ PolarScreen™ PPARγ-Competitor Assay Kit, Green

PolarScreen™ PPARγ-Competitor Assay Kit, Green

Supplier:  Invitrogen™ PV6136

Catalog No. PV6136

Description

The PPARγ (Peroxisome Proliferator-Activated Receptor-gamma) Competitor Assay, Green uses the human-derived recombinant PPARγ ligand-binding domain (PPARγ-LBD) tagged with an N-terminal GST-tag and a novel, tight-binding, selective fluorescent PPARγ ligand (Fluormone™ PPARγ Green) in a homogenous, mix-and-read assay format. PPARγ-LBD/Fluormone™ PPARγ Green complex produces high polarization. This complex is then added to individual test compounds in microwell plates. Competitors displace the fluorescent Fluormone™ PPARγ Green Ligand from the PPARγ-LBD/Fluormone™ PPARγ Green complex, causing the fluorescent ligand to tumble rapidly during its fluorescence lifetime, resulting in a low polarization value. Noncompetitors will not displace the fluorescent ligand from the complex, so the polarization value remains high. The shift in polarization value in the presence of the test compounds is used to determine relative affinity of test compounds for the PPARγ-LBD.

Specifications

• Color: Green
• For Use With (Equipment): Microplate Reader
• Content And Storage: PV3356 — Fluormone™ PPARγ Green (-20°C)
  PV4546 — PPARγ-LBD (-80°C)
  PV3358 — PPARγ Green Screening Buffer (room temperature)
  P2325 — 1M DTT (-80°C or -20°C)
  
• Excitation/Emission: 485/535
• Readout: End Point
• Target Entry: PPARG, PPAR gamma, NR1C3
• For Use With (Application): Competitive Binding Assay, Fluorescence Polarization
• Product Type: Assay Kit

• Detection Method: Fluorescent
• Packaging: 384-well Plate
• Assay Entry: Biochemical competitive binding
• Gene ID (Entrez): 5468
• Shipping Condition: Dry Ice
• Product Line: PolarScreen™
• Ligand: PPAR

For Research Use Only. Not for use in diagnostic procedures.