Invitrogen™ Platinum™ II Hot-Start Green PCR Master Mix (2X)

Invitrogen Platinum II Hot-Start Green PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup, as well as two tracking dyes for direct loading of PCR products on gels.

Catalog Number	Mfr. No.	No. of Reactions
14001012	Invitrogen™ 14001012	50 Reactions
14001013	Invitrogen™ 14001013	200 Reactions
14001014	Invitrogen™ 14001014

Description

Invitrogen Platinum II Hot-Start Green PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup, as well as two tracking dyes for direct loading of PCR products on gels. Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and leading hot-start technology.

Features of Platinum II Hot-Start Green PCR Master Mix (2X) include:

• Innovative buffer—enables universal annealing temperature by isostabilizing primer-template duplex structures
• Engineered Taq DNA polymerase—confers fast cycling and resistance to common inhibitors
• Platinum hot-start technology—enablessures superior specificity, sensitivity, and yields; allows for room temperature reaction setup
• Green PCR buffer—helps reduces pipetting error with direct gel loading

Platinum II Taq Hot-Start DNA Polymerase is an engineered Taq DNA polymerase that shows increased resistance to reaction inhibitors originating from sample material or DNA purification steps. The polymerase has a higher DNA synthesis rate and may deliver PCR results more than two times faster than other Taq DNA polymerases. Proprietary Platinum Taq antibodies block polymerase activity at ambient temperatures and dissociate after the initial denaturation step at 94°C. This automatic 'hot start' provides increased sensitivity, specificity, and yield, while allowing reaction assembly at room temperature.

Due to the unique composition of the Platinum II PCR buffer, the annealing temperature is 60°C for most primer pairs designed following the general design rules. Isostabilizing molecules in the buffer increase primer–template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize annealing temperature for each primer pair. With Platinum II Hot-Start Green PCR Master Mix (2X), different PCR assays can be cycled together using the same protocol with universal primer annealing temperature and the extension step selected for the longest fragment to be amplified.

Platinum II Hot-Start Green PCR Master Mix (2X) is provided with the optional Platinum GC Enhancer for specific amplification and improved yields of GC-rich targets.

Use Platinum II Taq Green Hot Start DNA Polymerase for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR, in applications like genotyping, high-throughput PCR, or with samples of suboptimal purity.

Platinum II Hot-Start PCR Master Mix (2X) (Cat. No. 14000012) is also available, which is the same master mix without the tracking dyes.

Specifications

• Format: Tube
• Concentration: 2X
• Hot Start: Built-In Hot Start

• GC-Rich PCR Performance: High
• Fidelity (vs. Taq): 1 X
• Size (Final Product): 5 kb or less

For Research Use Only