Invitrogen™ Platinum™ Direct PCR Universal Master Mix

Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA.

Supplier:  Invitrogen™ A44647500

Catalog No. A44647500

Description

Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA. It contains high-performing, engineered Platinum II Taq Hot-Start DNA Polymerase with dNTPs in an innovative buffer that enables universal primer annealing for superior performance in direct PCR applications.

Features of Platinum Direct PCR Universal Master Mix include:
• Direct DNA amplification from various samples due to engineered, inhibitor-tolerant Platinum II Taq Hot-Start DNA Polymerase
• Universal primer annealing temperature due to innovative buffer that enables primer annealing at 60°C
• Superior specificity, sensitivity, and yields due to Platinum hot-start technology

Platinum Direct PCR Universal Master Mix is designed to work with a variety of samples of different origins such as animal, human, plant, insect, worm, and bacterial samples. The kit includes optimized reagents to achieve superior results. The quick lysis protocol enables efficient amplification from templates up to 8 kb in size and co-cycling of different length fragments. Samples in the lysis buffer can be stored for later use. Platinum Direct PCR Universal Master Mix is provided with the optional Platinum GC Enhancer for specific amplification and improved yields of GC-rich targets.

Engineered Platinum II Taq DNA polymerase confers faster cycling and tolerance to reaction inhibitors originating from sample material. Proprietary Platinum Taq antibodies block polymerase activity at ambient temperatures and dissociate after the initial denaturation step. This automatic 'hot start' provides increased sensitivity, specificity, and yield, while allowing reaction assembly at room temperature.

Due to the unique composition of the Platinum Direct PCR Universal Master Mix, the annealing temperature of 60°C can be used for most primer pairs that follow the general design rules. Isostabilizing molecules in the buffer increase primer-template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize annealing temperature for each primer pair. Different PCR assays can be cycled together using the same protocol with a universal primer annealing temperature and an extension step selected for the longest fragment to be amplified.

Specifications

• Content And Storage: • 2X Platinum Direct PCR Universal Master Mix (5 mL)
  • Lysis Buffer (2 x 12.5 mL)
  • Proteinase K (0.75 mL)
  • Platinum GC Enhancer (2 x 1.25 mL)
  • Nuclease-free water (5 mL)
  
  Store at –20°C.
• Polymerase: Platinum II Taq Hot-Start DNA Polymerase
• Green Features: Less hazardous, less waste
• Shipping Condition: Dry Ice
• Concentration: 2X
• Hot Start: Built-In Hot Start
• Overhang: 3'-A
• Size (Final Product): 8 kb

• GC-Rich PCR Performance: High
• Reaction Speed: Fast
• Product Type: Direct PCR Universal Master Mix
• For Use With (Application): Hot-start PCR, Real Time PCR (qPCR)
• Fidelity (vs. Taq): 1X
• No. of Reactions: 500 Reactions
• Reaction Format: SuperMix or Master Mix