Thermo Scientific™ Sulfo-LC-SPDP (sulfosuccinimidyl 6-(3'-(2-pyridyldithio)propionamido)hexanoate)

Crosslink amines to sulfhydryls with this water-soluble sulfo-NHS-ester and pyridyldithiol groups on 10-atom spacer arms to form cleavable disulfide bonds with cysteines.

Supplier:  Thermo Scientific™ 21650

Catalog No. PI21650

Description

Thermo Scientific™ Pierce Sulfo-LC-SPDP is a water-soluble, long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteines.

Highlights:

• Forms cleavable disulfide bonds with sulfhydryls
• Releases a detectable byproduct after reacting with free sulfhydryl groups; by measuring the release of pyridine-2-thione at 343nm, the reaction progression can be monitored
• Reactive groups: sulfo-NHS ester and pyridyldisulfide
• Reactive toward: amino and sulfhydryl groups

Specifications

• Chemical Reactivity: Amine-Sulfhydryl
• Description: Sulfo-LC-SPDP
• PEGylated: No
• Content And Storage: Upon receipt store at -20°C protected from moisture.
• Shipping Condition: Ambient
• Labeling Method: Chemical Labeling
• Reactive Moiety: Pyridyldisulfide, Sulfo-NHS Ester
• Form: Powder
• Solubility: Water
• Water Soluble: Yes

• Cleavable: By Thiols
• Molecular Weight (g/mol): 527.57
• Spacer Arm Length: 15.7 Å
• Cell Permeability: No
• Product Line: Pierce™
• Crosslinker Type: Heterobifunctional
• Spacer: Medium (10 to 30 Å)
• Quantity: 50 mg
• Format: Standard
• Product Type: Crosslinker

For Research Use Only. Not for use in diagnostic procedures.