Thermo Scientific™ Pierce™ Sulfhydryl Addition Kit

Modify protein primary amines to create free sulfhydryl groups for labeling, crosslinking or immobilization techniques; complete kit with SATA reagent and hydroxylamine.

Supplier:  Thermo Scientific™ 23460

Catalog No. PI23460

Description

Thermo Scientific™ Pierce Sulfhydryl Addition Kit is a complete set of reagents, including SATA, to enable modification of primary amines to free sulfhydryl groups for use in heterobifunctional crosslinking techniques.

Highlights:

• Reacts with primary amines to add protected sulfhydryls
• An easy deprotection step generates a free sulfhydryl group
• Thermo Scientific™ Sulfhydryl Addition Kit contains all necessary components for thiolation, deprotection, purification and quantitation

Specifications

• Chemical Reactivity: Amine
• Label or Dye: Sulfhydryl Group
• Product Line: Pierce™
• Reactive Moiety: Active Ester, Succinimidyl Ester, NHS Ester
• Form: Powder
• Quantity: 1 Reaction
• Product Type: Crosslinker

• Content And Storage: Sufficient For: One modification experiment with 0.5 to 5 mg of purified protein
  • SATA, 2 mg
  • Hydroxylamine·HCl, 5 mg
  • Conjugation Buffer Stock (10X), 20 mL
  • PBS pack (makes 500 mL), 1 pack
  • Dimethylformamide (DMF), 1 mL
  • Dextran Desalting Column, 1 x 5 mL
  • Column Extender, 1
  • Ellman's Reagent (DTNB), 2 mg
  • Cysteine·HCl, 20 mg
  
  Store at 4°C.
• Label Type: Conjugating Groups
• Crosslinker Type: Heterobifunctional
• Spacer: Short (<10 Å)
• Reagent Type: SATA
• Solubility: DMF, DMSO

For Research Use Only. Not for use in diagnostic procedures.