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Thermo Scientific™ Pierce™ Streptavidin, Hydrazide-Activated

Perform Western blot, ELISA or other biotin-based detection with purified and HRP-, AP-, FITC-, TRITC-, PE- or APC-conjugated or maleimide-/hydrazide-activated streptavidin.

Supplier:  Thermo Scientific™ 21120

Catalog No. PI21120


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Description

Description

Thermo Scientific™ Pierce Streptavidin and Conjugates include purified recombinant streptavidin biotin-binding protein, plus standard SA-HRP, SA-AP and fluorescent conjugates for biotin-based assays.

These purified and conjugated forms of recombinant streptavidin protein enable detection of biotinylated antibodies and other probes in a variety of standard assay methods, including Western blotting, ELISA, immunohistochemistry and fluorescence imaging. Purified streptavidin and the streptavidin-HRP conjugates (peroxidase-conjugated streptavidin) are supplied lyophilized in phosphate-based buffers for immediate reconstitution with water. Alkaline Phosphatase conjugates are supplied as a liquid in a Tris-based buffer.

Highlights:

  • Recombinant streptavidin – high-quality protein, 53kDa, pI equals 6.8 to 7.5

  • Pure protein and enzyme conjugates – choose pure, unconjugated protein for biotin blocking and controls; enzyme conjugates for substrate-based detection

  • Fluorophore conjugates – standard fluorescent forms (fluorescein, rhodamine, etc.) for traditional immunofluorescence techniques

  • Activated preparations – choose a maleimide-activated or hydrazide-activated form to prepare a custom streptavidin conjugate with sulfhydryl- or carbohydrate-containing molecule

Specifications

Specifications

Streptavidin
2 mg
Ketone, Aldehyde
Soluble in Water
Pierce™
Streptavidin
Pierce Streptavidin, Hydrazide Activated
Storage: Upon receipt store at room temperature or 4°C. Product shipped at ambient temperature.
Amine, Hydrazide
Solid
Streptavidin Conjugate
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For Research Use Only. Not for use in diagnostic procedures.