Thermo Scientific™ Pierce™ Streptavidin Coated High Capacity Plates

Thermo Scientific™ Pierce Streptavidin High Binding Capacity (HBC) Coated Plates are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

Catalog Number	Mfr. No.	Description	Detection Method
PI15503	Thermo Scientific™ 15503	Black, 96-well	Fluorescence
PI15506	Thermo Scientific™ 15506	Black, 384-well	Fluorescence
PI15501	Thermo Scientific™ 15501	Clear, 8-well Strip	Colorimetric
PI15500	Thermo Scientific™ 15500	Clear, 96-well	Colorimetric
PI15504	Thermo Scientific™ 15504	Clear, 384-well	Colorimetric
PI15502	Thermo Scientific™ 15502	White, 96-well	Chemiluminescence
PI15505	Thermo Scientific™ 15505	White, 384-well	Chemiluminescence

Description

Pierce Streptavidin High Binding Capacity (HBC) Plates are prepared with an exclusive coating method that yields a streptavidin-coated plate with four to five times the binding capacity of other commercially available polystyrene assay plates. Using a Pierce Streptavidin HBC Plate can result in an assay with a broader dynamic range and better linearity, leading to improved assay precision. The plates are offered in several plate formats, plate colors, and coating volumes.

Thermo Scientific Pierce Streptavidin-Coated High-Capacity Plates are designed for the binding of biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

Pierce Streptavidin-coated plates are widely used for applications such as ELISAs and other assay protocols. These biotin-binding plates come with different capacities and sensitivities for maximum flexibility. For example, the high-binding-capacity plates (HBC) can be used for anti-therapeutic/anti-drug antibodies (ADA) screening for immunogenicity research and biologics development (1,2). Both regular and HBC plates have been used in aptamer assays for clinical and industrial needs, along with neurobiology research for therapeutics and prevention, amongst other applications (3–5).

Features of Streptavidin-Coated High-Capacity Plates include:
• Unique plate-coating technology—results in high loading and binding capacity
• Streptavidin-coated option—plates have up to five times the binding capacity of typical plates
• Broader dynamic range—extends the quantitative range so there is less need for dilutions
• Save time—pre-blocked plates to reduce the number of assay steps
• Flexible assay formats—coated plates offered in 8-well strips to reduce waste

The proprietary plate-coating technology used to manufacture these Pierce coated plates yields high-capacity plates with a wider detection limit than regular-binding-capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.

References
1. J Immunol Methods. 2016 Apr;431:45-51
2. J Immunol Methods. 2011 Feb 28;365(1-2):38-49
3. Cancers (Basel). 2022 Oct 25;14(21):5222
4. J Microbiol Biotechnol. 2013 Jun 28;23(6):878-84
5. Acta Neuropathol Commun. 2018 May 31;6(1):43

Specifications

• Binding Property: High Capacity, Affinity
• Product Line: Pierce
• Surface Treatment: Streptavidin
• Plate Blocking: SuperBlock™ Buffer

• Content And Storage: Upon receipt store plates at 4°C in unopened pouches. Once opened, place unused plates in a resealable bag with desiccant and store at 4°C.
• Material: Polystyrene
• No. per Pack: 5 Plates