Thermo Scientific™ Pierce™ Quantitative Peroxide Assay Kit (Lipid)

Detect and measure hydrogen peroxide levels (H2O2) in biological samples using an iron (Fe) and xylenol orange (XO) reagent for microplates or tubes.

Supplier:  Thermo Scientific™ 23285

Catalog No. PI23285

Description

Detect and measure hydrogen peroxide levels in biological samples using an iron and xylenol orange reagent with Thermo Scientific™ Pierce Quantitative Peroxide Assay Kits.

The Thermo Scientific Pierce Quantitative Peroxide Assay Kit (lipid-compatible formulation) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange (XO) reagent.

This lipid-compatible peroxide assay kit detects peroxides based on oxidation of ferrous to ferric ion in the presence of xylenol orange. The kit's formulation may be used with lipid-containing samples without first extracting the lipids; however, because this kit does not contain sorbitol, the assay is less sensitive. Most proteins do not interfere with this assay, although some metal chelators may require use of a blank (i.e., control) to mitigate these effects.

Features of the Quantitative Peroxide Assay Kit:
• Peroxides—detects and measures hydrogen peroxide (H2O2) levels in biological and other liquids samples
• Convenient—no proprietary reagents involved but the ready-to-use kit removes the variability, difficulty and added expense of formulating the reagents yourself

Applications:
• General hydrogen peroxide (H₂O₂) measurement in protein samples
• Quantitation of peroxides in detergents
• Assessment of the effects of peroxides on cellular activity
• Determination of protein glycation; Measurement of peroxide accumulation in lipids

In this assay, hydroperoxides convert the Fe2+ to Fe3+ at acidic pH. With this lipid-compatible formulation, the peroxide converts the Fe2+ to Fe3+ directly. In a sulfuric acid solution, the Fe3+ complexes with the xylenol orange (XO) dye to yield a purple product having an absorbance maximum at 560 nm. Peroxide levels in test samples can be determined by calculation from the known extinction coefficient of the XO-Fe complex or by reference to a standard curve prepared with hydrogen peroxide solution.

Related Products
Pierce™ Quantitative Peroxide Assay Kit, Aqueous (Cat. No. 23280)
Pierce™ Peroxide Assay Reagent A (Cat. No. 23281)
Pierce™ Peroxide Assay Reagent B (Cat. No. 23282)
Pierce™ Peroxide Assay Reagent C (Cat. No. 23283)

Specifications

• Content And Storage: Sufficient For: 100 tube assays or 500 microplate assays with lipid-containing samples
  • Reagent A, 1 mL
  • Reagent C, 4 x 25 mL
  
  Store at 4°C.
• Assay: Quantitative Peroxide Assay Kit
• Product Line: Pierce™
• Specificity: Not Target-Specific
• For Use With (Application): Solution-based Detection, Absorbance

• Description: Pierce Quantitative Peroxide Assay Kit (Lipid)
• Detection Method: Colorimetric
• Product Type: Quantitative Peroxide Assay
• Sufficient For: 100 Tube Assays or 500 Microplate Assays
• For Use With (Equipment): Spectrophotometer, Microplate Reader

For Research Use Only. Not for use in diagnostic procedures.