Thermo Scientific™ Pierce™ GST Agarose

Isolate or remove cross-reacting GST-specific serum or other antibody solutions with immobilized glutathione S-transferase agarose.

Supplier:  Thermo Scientific™ 20211

Catalog No. PI20211

Description

Perform isolation or removal of cross-reacting GST-specific antibodies from serum or other antibody solutions with Thermo Scientific™ Pierce GST Agarose.

This GST resin consists of purified glutathione S-transferase protein from Schistosoma japonicum that has been immobilized on crosslinked 6% beaded agarose. Antibody molecules in that cross-react with the GST portion of a recombinant fusion protein can be removed from the desired protein-specific antibody by passing antiserum over a GST agarose column. GST antibodies will bind to the immobilized GST, whereas antibodies against other fusion proteins will pass through the column unrestrained.

Highlights:

• Specific – provides purification of target antibodies from those directed against the GST tag

• Reusable – bound antibodies can be eluted with 6M guanidinium chloride allowing for 10 purifications with each column or aliquot of resin

• Two formats – choose either pre-packed columns or resin slurry for use at a variety of scales

Specifications

• Product Type: Agarose
• Column Type: Agarose Resin, Affinity
• Stationary Phase: GST Protein
• Description: Pierce GST Agarose
• Quantity: 5 mL

• Product Line: Pierce™
• Format: Bottle
• Purification Target: Anti-GST Antibody
• Content And Storage: 4°C

For Research Use Only. Not for use in diagnostic procedures.