Thermo Scientific™ Pierce™ Boronic Acid Resin

Selectively bind and remove or purify ribonucleotides and small oligonucleotide RNAs using polyacrylamide beads of immobilized m-aminophenyl-boronic acid.

Supplier:  Thermo Scientific™ 20244

Catalog No. PI20244

Description

Perform ribonucleotide and oligonucleotide RNA isolation with Thermo Scientific™ Pierce Boronic Acid Resin.

This immobilized boronic acid is an easy-to-use polyacrylamide affinity support for purification of ribonucleotides and other small molecular weight compounds that contain cis-diol groups. The ligand (m-aminophenyl-boronic acid) binds to the cis-diol groups on the sugar portion of nucleotides, forming a reversible five-member ring complex. After washing away non-bound molecules from the sample, the complex can be dissociated and the nucleotide eluted by lowering the pH or by addition of sorbitol.

Highlights:

• Ligand – boronic acid

• Support – beaded polyacrylamide (1800 MW exclusion limit)

• Spacer – m-aminophenyl group

• Loading – 100μmol boronate per milliliter of settled resin

• Capacity – at least 99% binding and recovery of 110μmol AMP per milliliter of settled resin

• Format – 50% resin slurry in 0.05% sodium azide

Specifications

• Content And Storage: Store at 4°C.
• Product Line: Pierce™
• Target: Ribonucleotide
• Stationary Phase: Boronic Acid
• Column Type: Affinity, Polyacrylamide Resin

• Form: Resin Slurry
• Quantity: 10 mL
• Type: Resin
• Format: Bottle

For Research Use Only. Not for use in diagnostic procedures.