Thermo Scientific™ Pierce™ BCA Protein Assay Reagent A

Estimate visually, or measure with a standard spectrophotometer or plate reader at 562 nm.

Supplier:  Thermo Scientific™ 23221

Catalog No. PI23221

Description

Thermo Scientific™ Pierce BCA Protein Assay Kit remains one of the most popular protein quantitation methods worldwide.

Additional Information and Online Ordering

• A component of the Pierce BCA Protein Assay Kit
• Kit is a two-component, high-precision, detergent-compatible assay that is used for total protein concentration determination compared to a protein standard
• Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research
• The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications
• Compared to most dye-binding methods, the BCA assay is affected much less by protein compositional differences, providing greater concentration accuracy
• Estimate visually or measure with a standard spectrophotometer or plate reader at 562 nm
• Exhibits less protein-to-protein variation than dye-binding methods (Bradford)
• Unaffected by typical concentrations of most ionic and non-ionic detergents
• Assay has a 30 min. incubation; much easier and four times faster than classical Lowry methods
• Linear working range for BSA of 20 to 2000 μg/mL
• Detect down to 5 μg/mL with the enhanced protocol
• Store at room temperature

Specifications

• Content And Storage: Store at room temperature.
• Assay: BCA Assay
• Product Line: Pierce™
• Specificity: Not Target-Specific
• For Use With (Equipment): Spectrophotometer, Microplate Reader

• Description: Pierce BCA Protein Assay Reagent A
• Detection Method: Colorimetric
• Product Type: Protein Quantitation Assay
• For Use With (Application): Solution-based Detection, Absorbance
• Quantity: 250 mL

For Research Use Only. Not for use in diagnostic procedures.