Thermo Scientific™ Phusion™ HF and GC Buffer Packs

5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.

Supplier:  Thermo Scientific™ F538L

Catalog No. F538L

Includes: Phusion HF Buffer Pack

• 5x Phusion HF Buffer (1X buffer provides 1.5 mM MgCl₂)
• 50 mM MgCl₂ solution
• DMSO

Phusion Green HF Buffer Pack

• 5X Phusion Green HF Buffer (1X buffer provides 1.5 mM MgCl₂)
• 50 mM MgCl₂ solution
• DMSO

Description

The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10^-7) is lower than that in GC Buffer (9.5 x 10^-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:

• 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
• 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))

Specifications

• Content And Storage: • 5X Phusion Green HF Reaction Buffer (4 x 1.5 mL)
  • 50 mM MgCl₂ (4 x 1.5 mL)
  • DMSO (0.5 mL)
  
  Store at –20°C.
• Product Type: HF Buffer Pack
• For Use With (Application): Standard PCR, High-fidelity PCR
• Volume: 4 x 1.5 mL

• Polymerase: Phusion High-Fidelity DNA Polymerase
• Shipping Condition: Wet or Dry Ice
• Starting Material: DNA
• Color: Green

For Research Use Only. Not for use in diagnostic procedures.