Phosphotyrosine Monoclonal Antibody (pY20), Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen 037700

Catalog No. 03-770-0

Description

This antibody detects proteins which contain phosphorylated tyrosine residues. This antibody is known to show cross-reactivity towards phosphothreonine.

The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.

Specifications

• Antigen: Phosphotyrosine
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.1% sodium azide; pH 7.4
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Target Species: Chemical
• Product Type: Antibody
• Isotype: IgG2b

• Applications: ELISA, Immunoprecipitation, Western Blot
• Clone: pY20
• Conjugate: Unconjugated
• Gene Alias: Phosphotyrosine; pTyr; pY
• Immunogen: Keyhole limpet hemocyanin coupled with equal amounts of phosphotyrosine, glycine, and alanine
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: -20°C
• Form: Liquid
• Target Molecule: Chemical